| Academic Year |
2026Year |
School/Graduate School |
Graduate School of Biomedical and Health Sciences (Doctoral Course) |
| Lecture Code |
TB919403 |
Subject Classification |
Specialized Education |
| Subject Name |
ゲノム編集演習 |
Subject Name
（Katakana） |
ゲノムヘンシュウエンシュウ |
Subject Name in
English |
Genome Editing Coursework |
| Instructor |
AGO YUKIO,AOTO KAZUSHI,YOSHIMOTO TETSUYA,KATO FUMINORI |
Instructor
(Katakana) |
アゴウ ユキオ,アオト カズシ,ヨシモト テツヤ,カトウ フミノリ |
| Campus |
Kasumi |
Semester/Term |
1st-Year, Second Semester, Second Semester |
| Days, Periods, and Classrooms |
(2nd) Inte |
| Lesson Style |
Seminar |
Lesson Style
(More Details) |
Face-to-face |
| Lectures, exercises, practical training, discussions, student presentations, assignments, the use of chemicals |
| Credits |
2.0 |
Class Hours/Week |
|
Language of Instruction |
B
:
Japanese／English |
| Course Level |
6
:
Graduate Advanced
|
| Course Area（Area） |
26
:
Biological and Life Sciences |
| Course Area（Discipline） |
04
:
Life Sciences |
| Eligible Students |
Doctoral program |
| Keywords |
Genome editing using guide RNA/Cas9, mutant cell line/mouse, protein structure prediction, gene therapy |
| Special Subject for Teacher Education |
|
Special Subject |
|
Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) | |
|---|
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) | |
|---|
Class Objectives
/Class Outline |
Lectures, computer-based exercises, and laboratory experiments will provide students with fundamental knowledge and practical skills in genome editing technologies targeting cells and mice. |
| Class Schedule |
Lesson 1: Coursework guidance
Lesson 2: Gene and protein analysis tool “Benchiling”, Cloning method
Lesson 3: Creation of a genome map for genome editing
Lesson 4: Design of knockout cells via genome editing
Lesson 5: Design of knock-in cells via genome editing
Lesson 6: Design of knockout mouse via genome editing
Lesson 7: Design of knock-in mouse via genome editing
Lesson 8: Protein structure prediction using Alphafold2/3 and design of Tag proteins
Lesson 9: Fundamentals of gene therapy using genome editing
Lesson 10: Cell culture, gene delivery of genome editing vectors into cells
Lesson 11: Genotyping by PCR
Lesson 12: Electrophoresis
Lesson 13: Sanger sequence
Lesson 14: Analysis of sequence results
Lesson 15: Coursework summary, reserve day |
Text/Reference
Books,etc. |
Information will be provided in class as appropriate. |
PC or AV used in
Class,etc. |
Text, Handouts, Other (see [More Details]) |
| (More Details) |
PC |
| Learning techniques to be incorporated |
Discussions, Post-class Report |
Suggestions on
Preparation and
Review |
Assignments related to the computer-based exercises will be provided because class time alone may be insufficient for fully consolidating the knowledge and skills.
Please complete the assignments and review the content covered in each session. |
| Requirements |
In principle, classes will be held every Tuesday from 6:00 PM to 7:30 PM.
The schedule may be adjusted depending on the student's situation and the content of the class. |
| Grading Method |
Grades will be determined based on overall evaluation of class participation (attendance and engagement) and the level of achievement on assignments for the computer-based exercises. |
| Practical Experience |
|
| Summary of Practical Experience and Class Contents based on it |
|
| Message |
|
| Other |
Please bring your own laptop computer for the hands-on computer exercises. |
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. |