| Academic Year |
2024Year |
School/Graduate School |
Graduate School of Integrated Sciences for Life (Master's Course) Division of Integrated Sciences for Life Program of Mathematical and Life Sciences |
| Lecture Code |
WG124002 |
Subject Classification |
Specialized Education |
| Subject Name |
プロテオミクス |
Subject Name
（Katakana） |
プロテオミクス |
Subject Name in
English |
Proteomics |
| Instructor |
KATAYANAGI KATSUO,OOMAE EIJI |
Instructor
(Katakana) |
カタヤナギ カツオ,オオマエ エイジ |
| Campus |
Higashi-Hiroshima |
Semester/Term |
1st-Year, First Semester, 2Term |
| Days, Periods, and Classrooms |
(2T) Mon5-6,Weds3-4：Online |
| Lesson Style |
Lecture |
Lesson Style
(More Details) |
|
| By lerning how to obtain protein structure, structure data is able to utilize for the research. |
| Credits |
2.0 |
Class Hours/Week |
|
Language of Instruction |
J
:
Japanese |
| Course Level |
6
:
Graduate Advanced
|
| Course Area（Area） |
25
:
Science and Technology |
| Course Area（Discipline） |
07
:
Chemistry |
| Eligible Students |
|
| Keywords |
Protein Crystallography, solution structure, and Biochemical Function |
| Special Subject for Teacher Education |
|
Special Subject |
|
Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) | |
|---|
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) | |
|---|
Class Objectives
/Class Outline |
Protein Crystallography is the center of the proteomics. This lecture introduces the principle of x-ray crystallograpy as well as how to utilize to structural biology. Moreover,
the solution structure by several spectroscopies are introduced. |
| Class Schedule |
1. The role of protein x-ray crystallography in proteomics
2. Spectroscopy for proteins: 1. Absorption and fluorescence.
3. Spectroscopy for proteins: 2. Circular dichroism (CD) and Nuclear Magnetic Resonance (NMR)
4. The protein function approached by protein crystallograpy
5. Electron diffraction and Neutron diffraction.
6. Solution structure by X-ray small angle scattering
7. The evaluation of x-ray crystallographic data on the paper.
8. The base of x-ray diffraction 1: Phases
9. The base of x-ray diffraction 2: Structure Factor
10. The base of x-ray diffraction 3: Reciprocal lattice
11. The base of x-ray diffraction 4: Ewald's sphere
12. The symmetry of the crystal
13. The symmetry of the diffraction pattern and the extinction rule
14. Protein crystallogtaphy by synchrotron radiation, and time resolved structure
15. Review |
Text/Reference
Books,etc. |
Blundell & Johnson "Protein Crystallography" Academic Press
Max Perutz "Protein Structure" W.H.Freeman and Company
John R.Helliwell "Macromolecular Crystallography with Synchrotron Radiation" Cambridge University Press |
PC or AV used in
Class,etc. |
|
| (More Details) |
Projector |
| Learning techniques to be incorporated |
|
Suggestions on
Preparation and
Review |
Prints are provided as a tool to help comprehension. |
| Requirements |
Attendance and reports are used for the evaluation. |
| Grading Method |
Reports |
| Practical Experience |
|
| Summary of Practical Experience and Class Contents based on it |
|
| Message |
This research field is the mixture of physics, chemistry and biology. |
| Other |
|
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. |