| Class Schedule |
There are eight lectures.
1 [Group A: Wed, April 10th, 9:30-12:30], [Group B: Fri, April 12th, 9:30-12:30]
"Review of molecular biology"
Students will understand DNA,RNA and protein structures, molecular genetics and the biochemistry of transcription and protein synthesis. Each student receives the assignment from Teams and completes it at home or in the lab. You are required to provide your laptop and writing implements.
2 [Group A: Wed, April 17th, 9:30-12:30], [Group B: Fri, April 19th, 9:30-12:30]
"Transfection DNA Plasmid into Mammalian Cells”
Students can carry out basic cell-culture and transfection techniques properly and safely on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.
3 [Group A: Tue, April 23rd, 9:30-15:30], [Group B: Thu, April 25th, 9:30-15:30]
"Protein gel electrophoresis part1”
Students will learn about principle and technique of protein gel electrophoresis using SDS-PAGE. In addition, they will set up gels and perform on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.
4 [Group A: Wed, April 24th, 9:30-12:30], [Group B: Fri, April 26th, 9:30-12:30]
"Protein gel electrophoresis part2”
Students will detect targeted protein expression using ECL reagents with the ChemiDoc imaging system on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.
5 [Group A: Wed, May 8th, 9:30-12:30], [Group B: Fri, May 10th, 9:30-12:30]
"Purification of nucleic acid”
Students will learn about principle and technique of nucleic acid purification, reverse transcription and cDNA synthesis on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.
6 [Group A: Wed, May 15th, 9:30-12:30], [Group B: Fri, May 17th, 9:30-17:00]
"PCR, agarose gel electrophoresis”
Students will design primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.
7 [Group A: Wed, May 22nd, 9:30-12:30], [Group B: Fri, May 24th, 9:30-12:30]
"Real time PCR”
Students will quantify the expression levels of the target gene and endogenous control gene by real time PCR. on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.
8 [Group A: Wed, May 29th, 9:30-12:30], [Group B: Fri, May 31st, 9:30-12:30]
"Data analysis and review of the class”
Students will learn about principle and technique of mRNA expression level quantification by real time PCR. Each student receives the assignment from Teams and completes it at home or in the lab. You are required to provide your laptop and writing implements.
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