Hiroshima University Syllabus

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Academic Year 2024Year School/Graduate School School of Pharmaceutical Sciences Program of Pharmaceutical Sciences
Lecture Code I7097001 Subject Classification Specialized Education
Subject Name 生物化学実習
Subject Name
Subject Name in
Experiments of Biological Chemistry
ノムラ ワタル,ニゴリカワ キヨミ,マツモト ダイスケ
Campus Kasumi Semester/Term 2nd-Year,  Second Semester,  Second Semester
Days, Periods, and Classrooms (2nd) Inte
Lesson Style Practical Lesson Style
(More Details)
Credits 1.0 Class Hours/Week   Language of Instruction B : Japanese/English
Course Level 2 : Undergraduate Low-Intermediate
Course Area(Area) 27 : Health Sciences
Course Area(Discipline) 03 : Pharmaceutical Sciences
Eligible Students
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
(Applicable only to targeted subjects for undergraduate students)
Pharmaceutical Sciences
(Abilities and Skills)
・To be able to handle major analysis methods written in the Japanese Pharmacopoeia. ●quality ⑤

Medicinal Sciences
(Knowledge and Understanding)
・Knowledge of human and biological bodies.
(Abilities and Skills)
・Development of knowledge about human and biological bodies. (advance)
・To be able to measure and evaluate major biological reactions.
(Comprehensive Abilities)
・1. The active attitude of dealing with issues on drug development and environmental hygiene.  2. The social responsibility as a specialist of drug development and environmental hygiene.  3. The comprehensive, scientific and calm attitude to solve problems.  4. The cooperative attitude in team research.  5. The ability to make communication and presentation.  6. The ability of assessment and analysis.  7. The active usage of information technology and the management ability.  8. The ethical consideration toward genetically modified foods and animal experiments 
Class Objectives
/Class Outline
To obtain fundamental techniques for biochemistry 
Class Schedule 1. Enzyme kinetics
   Determination of Km and Vmax
2-1. Plasmid isolation using Alkaline Lysis Method and Midiprep Kit
2-2. Transfection of 293A cells with pEGFP-PKCdelta
2-3. Monitoring of PKCdelta-EGFP using fluorescense microscopy
3-1. In vitro validation of gene editing by CRISPR-Cas9
3-2. Transfection of 293A cells with hCas9 and gRNA
3-3. Isolation of genomic DNA from 293A cells
3-4. Validation of genome editing using T7E1 assay 
PC or AV used in
(More Details) Bring the following items to the class:
   protection glasses, laptop 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Grading Method Attitudes and Behavior 30 points; Experimental Skills 20 points; Quality of the submitted report paper 50 points. Total 100points. The minimum passing grade is 60 points. 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Other SBOs: C6-(2)-⑧-1,C6-(3)-③-1,C6-(3)-③-2,C6-(3)-③-3,C6-(3)-③-4 
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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