| Academic Year |
2024Year |
School/Graduate School |
School of Pharmaceutical Sciences Program of Pharmaceutical Sciences |
| Lecture Code |
I7097001 |
Subject Classification |
Specialized Education |
| Subject Name |
生物化学実習 |
Subject Name
（Katakana） |
セイブツカガクジッシュウ |
Subject Name in
English |
Experiments of Biological Chemistry |
| Instructor |
NOMURA WATARU,NIGORIKAWA KIYOMI,MATSUMOTO DAISUKE |
Instructor
(Katakana) |
ノムラ ワタル,ニゴリカワ キヨミ,マツモト ダイスケ |
| Campus |
Kasumi |
Semester/Term |
2nd-Year, Second Semester, Second Semester |
| Days, Periods, and Classrooms |
(2nd) Inte |
| Lesson Style |
Practical |
Lesson Style
(More Details) |
|
| Experiments |
| Credits |
1.0 |
Class Hours/Week |
|
Language of Instruction |
B
:
Japanese／English |
| Course Level |
2
:
Undergraduate Low-Intermediate
|
| Course Area（Area） |
27
:
Health Sciences |
| Course Area（Discipline） |
03
:
Pharmaceutical Sciences |
| Eligible Students |
|
| Keywords |
|
| Special Subject for Teacher Education |
|
Special Subject |
|
Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) | |
|---|
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) | Pharmaceutical Sciences
（Abilities and Skills）
・To be able to handle major analysis methods written in the Japanese Pharmacopoeia. ●quality ⑤
Medicinal Sciences
（Knowledge and Understanding）
・Knowledge of human and biological bodies.
（Abilities and Skills）
・Development of knowledge about human and biological bodies. (advance)
・To be able to measure and evaluate major biological reactions.
（Comprehensive Abilities）
・1. The active attitude of dealing with issues on drug development and environmental hygiene. 2. The social responsibility as a specialist of drug development and environmental hygiene. 3. The comprehensive, scientific and calm attitude to solve problems. 4. The cooperative attitude in team research. 5. The ability to make communication and presentation. 6. The ability of assessment and analysis. 7. The active usage of information technology and the management ability. 8. The ethical consideration toward genetically modified foods and animal experiments |
|---|
Class Objectives
/Class Outline |
To obtain fundamental techniques for biochemistry |
| Class Schedule |
1. Enzyme kinetics
Determination of Km and Vmax
2-1. Plasmid isolation using Alkaline Lysis Method and Midiprep Kit
2-2. Transfection of 293A cells with pEGFP-PKCdelta
2-3. Monitoring of PKCdelta-EGFP using fluorescense microscopy
3-1. In vitro validation of gene editing by CRISPR-Cas9
3-2. Transfection of 293A cells with hCas9 and gRNA
3-3. Isolation of genomic DNA from 293A cells
3-4. Validation of genome editing using T7E1 assay |
Text/Reference
Books,etc. |
Handouts |
PC or AV used in
Class,etc. |
|
| (More Details) |
Bring the following items to the class:
protection glasses, laptop |
| Learning techniques to be incorporated |
|
Suggestions on
Preparation and
Review |
... |
| Requirements |
|
| Grading Method |
Attitudes and Behavior 30 points; Experimental Skills 20 points; Quality of the submitted report paper 50 points. Total 100points. The minimum passing grade is 60 points. |
| Practical Experience |
|
| Summary of Practical Experience and Class Contents based on it |
|
| Message |
|
| Other |
SBOs: C6-(2)-⑧-1，C6-(3)-③-1，C6-(3)-③-2，C6-(3)-③-3，C6-(3)-③-4 |
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. |