Hiroshima University Syllabus

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Academic Year 2024Year School/Graduate School School of Applied Biological Science
Lecture Code L5020006 Subject Classification Specialized Education
Subject Name 生命物質分析学
Subject Name
Subject Name in
Bio-Analytical Science
オキナカ ヤスシ,イクタニ マサシ,タナカ ワカナ,ナカエ ススム,サンボンギ ヨシヒロ,マツザキ メイ,フジイ ソウタロウ,ニシボリ マサヒデ,ホリウチ ヒロユキ,シマダ マサユキ,フナト コウイチ,フジカワ ユキチ,オオムラ ヒサシ,トミナガ ジュン
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  4Term
Days, Periods, and Classrooms (4T) Tues1-4:ABS C302
Lesson Style Lecture Lesson Style
(More Details)
The lecture content is quite specialized and may be difficult to understand in one face-to-face class, so please study repeatedly on demand. 
Credits 2.0 Class Hours/Week   Language of Instruction J : Japanese
Course Level 4 : Undergraduate Advanced
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 01 : Agricultural Sciences
Eligible Students The required subject of the Molecular Agro-Life Science Program
Keywords Check keywords in the Class Schedule below 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
(Applicable only to targeted subjects for undergraduate students)
Molecular Agro-Life Science Program
(Knowledge and Understanding)
・Knowledge and understanding regarding organic molecule, cell, individual organism, and population 
Class Objectives
/Class Outline
Students will learn about methodology applied to innovative technology development in the area of food resource production and healthcare.
Lecture will be provided by 15 faculty members of the Molecular Agro-Life Science Program, 
Class Schedule Lesson1: Omura H.
Title: Chromatography (HPLC and GC)
Keywords: Separation of mixture sample, Column, Detector, Retention

Lesson2: Tominaga J.
Title: Plant Research by Isotope Analysis
Keywords: Isotopes, Determination

Lesson3: Matuzaki M.
Title: Statistical Analysis and Statistical Modeling of Life Science Data
Keywords: Statistics, Statistical Models, R

Lesson4: Fujii S.
Title: Analysis of Protein
Keywords: Cell disruption, Chromatography, Electrophoresis, Quantity Determination, Crystallization and X-ray Diffraction Analysis

Lesson5: Fujikawa Y.
Title: Protein-protein Interaction Assay
Keywords: Immunoprecipitation, Yeast Two Hybrid、Fluorescence Resonance Energy Transfer, Bimolecular Fluorescence Complementation

Lesson6: Horiuchi H.
Title: Microscope
Keywords: Engineering Principle, Types, Observation Methods

Lesson7: Ikutani M.
Title: Analysis of Cell Populations with Fluorescent Materials
Keywords: Flow Cytometry Method (FACS), Immunostaining Method, FACS Sorting

Lesson8: Nakae S.
Title: Quantification of Bioactive Substances
Keywords: ELISA, EIA, ELISPOT, Multiplex, Antibody, scRNAseq

Lesson9: Shimada M.
Title: Cell Differentiation and Epigenetics
Keywords: Gene Expression, DNA Methylation Analysis, Chromatin Immunoprecipitation

Lesson10: Okinaka Y.
Title: Quantification of Microorganisms
Keywords: Eukaryotes, Prokaryotes, Viruses

Lesson11: Funato K.
Title: Forward Genetic Analysis Using Model Organisms
Keywords: Forward Genetics, Reverse Genetics, Mutation, Screening, Responsible Gene

Lesson12: Tanaka W.
Title: Methods in Plant Molecular Genetics
Keywords: Gene Expression Analysis、Mutant Analysis、Observation of Plant Organs and Tissues、Generation of Transgenic Plant

Lesson13: Sambongi Y.
Title: Biology Leading to Bio-Analytical Science
Keywords: Microorganism Screening Method, Culture Accumulation Method, Thermophilic Bacteria Protein Analysis Method, Analysis of Biological Materials from Animal Behavior

Lesson 14: Nishibori M.
Title: Analysis Methods for Animal Genome Evolution
Keywords: Phenotype and Genotype, Phylogenetic Tree, Gene Tree, Genomic Evolution, Molecular Phylogenetics
Lesson 15: Reconfirmation of learning
This is the time to review all the content up to this point and to inquire with each instructor about anything you do not understand.

There is a short examination or a report at each lesson. 
There is no prescribed text book.
PC or AV used in
(More Details) Handouts, Projector, Whitebord (Blackbord) 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Before taking each lesson, study the meaning of keywords.
After each lesson, review the lesson.
Requirements Each lesson will be provided by different faculty members.
There is a short examination or a report at each lesson. 
Grading Method There is a short examination or a report at each lesson, although there is no examination at the end of an academic term. The grade of students will be evaluated with the score of short tests and report assignment.
According to university regulations, students who are absent for more than a third of the total lessons will not be able to take the final exam.  
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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