L5008004 Laboratory Work in Molecular Agro-life ScienceIII

Hiroshima University Syllabus

Japanese

Academic Year 2024Year School/Graduate School School of Applied Biological Science

Lecture Code L5008004 Subject Classification Specialized Education

Subject Name 分子農学生命科学実験III

Subject Name
（Katakana）ブンシノウガクセイメイカガクジッケンIII

Subject Name in
English Laboratory Work in Molecular Agro-life ScienceIII

Instructor FUNATO KOUICHI,MATSUZAKI MEI,IKEDA ATSUKO,HORIUCHI HIROYUKI

Instructor
(Katakana) フナト　コウイチ,マツザキ　メイ,イケダ　アツコ,ホリウチ　ヒロユキ

Campus Higashi-Hiroshima Semester/Term 2nd-Year, Second Semester, Intensive

Days, Periods, and Classrooms (Int) Inte

Lesson Style Experiment Lesson Style
(More Details) 　

Experiment

Credits 1.0 Class Hours/Week 　 Language of Instruction J : Japanese

Course Level 2 : Undergraduate Low-Intermediate

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 01 : Agricultural Sciences

Eligible Students

Keywords cell engineering, cell culture, transfection, centrifugation, cell fractionation, microscopic observation, cell/organelle staining

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) Molecular Agro-Life Science Program
（Abilities and Skills）
・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population

Class Objectives
/Class Outline The experimental basis to analyze cells is learned.

Class Schedule lesson1
Guidance and cell culture
lesson2
Introduction of DNA into cells (yeast)
lesson3
Colony PCR for confirmation of gene knockout
lesson4
Isolation of knockout cells and observation of fluorescent proteins
lesson5
Understanding of reagents and laboratory equipment for animal cells
lesson6
Separation of several cells in blood
lesson7
Immunostaining of cultured cells
lesson8
Observation of animal tissues
lesson9
lesson10
lesson11
lesson12
lesson13
lesson14
lesson15

Text/Reference
Books,etc. A textbook will be distributed.

PC or AV used in
Class,etc. 　

(More Details) centrifuge, autoclave, fluorescence microscope, invert microscope, etc

Learning techniques to be incorporated 　

Suggestions on
Preparation and
Review Read the textbook, which will be distributed on the first day, and write and submit a report.

Requirements

Grading Method Experiments and reports

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message

Other

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2024Year	School/Graduate School	School of Applied Biological Science
Lecture Code	L5008004	Subject Classification	Specialized Education
Subject Name	分子農学生命科学実験III
Subject Name （Katakana）	ブンシノウガクセイメイカガクジッケンIII
Subject Name in English	Laboratory Work in Molecular Agro-life ScienceIII
Instructor	FUNATO KOUICHI,MATSUZAKI MEI,IKEDA ATSUKO,HORIUCHI HIROYUKI
Instructor (Katakana)	フナト　コウイチ,マツザキ　メイ,イケダ　アツコ,ホリウチ　ヒロユキ
Campus	Higashi-Hiroshima	Semester/Term	2nd-Year, Second Semester, Intensive
Days, Periods, and Classrooms	(Int) Inte
Lesson Style	Experiment	Lesson Style (More Details)
Experiment
Credits	1.0	Class Hours/Week		Language of Instruction	J : Japanese
Course Level	2 : Undergraduate Low-Intermediate
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	01 : Agricultural Sciences
Eligible Students
Keywords	cell engineering, cell culture, transfection, centrifugation, cell fractionation, microscopic observation, cell/organelle staining
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Molecular Agro-Life Science Program （Abilities and Skills）・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population
Class Objectives /Class Outline	The experimental basis to analyze cells is learned.
Class Schedule	lesson1 Guidance and cell culture lesson2 Introduction of DNA into cells (yeast) lesson3 Colony PCR for confirmation of gene knockout lesson4 Isolation of knockout cells and observation of fluorescent proteins lesson5 Understanding of reagents and laboratory equipment for animal cells lesson6 Separation of several cells in blood lesson7 Immunostaining of cultured cells lesson8 Observation of animal tissues lesson9 lesson10 lesson11 lesson12 lesson13 lesson14 lesson15
Text/Reference Books,etc.	A textbook will be distributed.
PC or AV used in Class,etc.
(More Details)	centrifuge, autoclave, fluorescence microscope, invert microscope, etc
Learning techniques to be incorporated
Suggestions on Preparation and Review	Read the textbook, which will be distributed on the first day, and write and submit a report.
Requirements
Grading Method	Experiments and reports
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message
Other
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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