L5006004 Laboratory Work in Molecular Agro-life ScienceII

Hiroshima University Syllabus

Japanese

Academic Year 2024Year School/Graduate School School of Applied Biological Science

Lecture Code L5006004 Subject Classification Specialized Education

Subject Name 分子農学生命科学実験II

Subject Name
（Katakana）ブンシノウガクセイメイカガクジッケンII

Subject Name in
English Laboratory Work in Molecular Agro-life ScienceII

Instructor TANAKA WAKANA,FUJIKAWA YUKICHI,TOMINAGA JUN

Instructor
(Katakana) タナカ　ワカナ,フジカワ　ユキチ,トミナガ　ジュン

Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  Intensive

Days, Periods, and Classrooms (Int) Inte

Lesson Style Experiment Lesson Style
(More Details) 　

Molecular Biological experiments.

Credits 1.0 Class Hours/Week 　 Language of Instruction J : Japanese

Course Level 2 : Undergraduate Low-Intermediate

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 01 : Agricultural Sciences

Eligible Students

Keywords DNA isolation, PCR, Electrophoretic detection, PCR extraction, Restriction Enzyme treatment, ligation reaction, transformation

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) Molecular Agro-Life Science Program
（Abilities and Skills）
・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population

Class Objectives
/Class Outline The main aim of this class is to learn experimental techniques in molecular biology.

Class Schedule lesson1: Plant genetics experiment (Genotyping of rice plants) 1（Tanaka）
　　- DNA isolation from plant tissue
　　- Leaf sampling
　　- DNA  isolation by alcohol precipitation
lesson2: Plant genetics experiment (Genotyping of rice plants) 2（Tanaka）
　　- Amplification of a target gene by PCR reaction
lesson3: Plant genetics experiment (Genotyping of rice plants) 3（Tanaka）
　　- Detection of PCR products by agarose gel electrophoresis
　　- Identification of a mutation
lesson4: Plant genetics experiment (Genotyping of rice plants) 4（Tanaka）
　　- Summary of Parts 1 to 3
　　- Writing a report
lesson 5: Subcloning 1 (Fujikawa)
　　- Plasmid extraction from Escherichia coli
lesson 6: Subcloning 2 (Fujikawa)
　　- Cutting and recombination of plasmids
　　-Confirmation of extracted plasmids
lesson 7: Subcloning 3 (Fujikawa)
　　- Confirmation of cut plasmids
　　-Transformation of Escherichia coli
lesson 8: Subcloning 4 (Fujikawa)
　　- Observation of colonies
　　-Report writing from Session 5 to Session 8
lesson9: Plant ecophysiology experiment 1（Tominaga）
    - Measuremetns of photosynthesis in leaves in laboratories
lesson10: Plant ecophysiology experiment 2（Tominaga）
　　- Measuremetns of photosynthesis in leaves in fields
lesson11: Plant ecophysiology experiment 3（Tominaga）
　　- Measuremetns of photosynthesis in leaves in fields
lesson12:  Plant ecophysiology experiment 4（Tominaga）
　　- Summary of Parts 1 to 3
　　- Writing a report

Reports are assigned according to the instructor in charge of the practical.
After each experiment conducted by each instructor, submit an experiment report according to the instructions of the instructor.
Lessions 1-4: Tanaka
Lessions 5-8: Fujikawa
Lessions 9-12: Tominaga

The above schedule is tentative. Changes may occur depending on the progress of the experiment, etc.

Text/Reference
Books,etc. Textbook: A textbook for experiment will be given.
Reference books:
Bio-Experiment Illustrated 1: Basics of Molecular Biology Experiments (Cellular Engineering Supplement, Shujunsha)
Experimental protocols for model plants: Rice, Arabidopsis, and Miyakogusa (Cellular Engineering Supplement, Shujunsha)
Measurement and modelling of photosynthesis and  production in plants (Kyouritsu shuppan)

PC or AV used in
Class,etc. 　

(More Details) Experimental equipments. PC.

Learning techniques to be incorporated 　

Suggestions on
Preparation and
Review Read the textbook for experiment.
Organize experiment records after each experiment.
Also, individually further analyze the discussed contents.
Prepare for the next experiment based on the distributed text.
For sessions 5 to 8, be sure to take a comprehension test before starting the 5th session.

Requirements

Grading Method Report(60%) &  attitude during experiment(40%).
However, according to the university regulations, if more than one-third of the classes are absent without permission, the performance cannot be evaluated.

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message Always prepare an experiment notebook and bring a PC.
This practical will be conducted by three instructors (Tanaka, Fujikawa, Tominaga).

Other

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2024Year	School/Graduate School	School of Applied Biological Science
Lecture Code	L5006004	Subject Classification	Specialized Education
Subject Name	分子農学生命科学実験II
Subject Name （Katakana）	ブンシノウガクセイメイカガクジッケンII
Subject Name in English	Laboratory Work in Molecular Agro-life ScienceII
Instructor	TANAKA WAKANA,FUJIKAWA YUKICHI,TOMINAGA JUN
Instructor (Katakana)	タナカ　ワカナ,フジカワ　ユキチ,トミナガ　ジュン
Campus	Higashi-Hiroshima	Semester/Term	2nd-Year, Second Semester, Intensive
Days, Periods, and Classrooms	(Int) Inte
Lesson Style	Experiment	Lesson Style (More Details)
Molecular Biological experiments.
Credits	1.0	Class Hours/Week		Language of Instruction	J : Japanese
Course Level	2 : Undergraduate Low-Intermediate
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	01 : Agricultural Sciences
Eligible Students
Keywords	DNA isolation, PCR, Electrophoretic detection, PCR extraction, Restriction Enzyme treatment, ligation reaction, transformation
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Molecular Agro-Life Science Program （Abilities and Skills）・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population
Class Objectives /Class Outline	The main aim of this class is to learn experimental techniques in molecular biology.
Class Schedule	lesson1: Plant genetics experiment (Genotyping of rice plants) 1（Tanaka）　　- DNA isolation from plant tissue 　　- Leaf sampling 　　- DNA isolation by alcohol precipitation lesson2: Plant genetics experiment (Genotyping of rice plants) 2（Tanaka）　　- Amplification of a target gene by PCR reaction lesson3: Plant genetics experiment (Genotyping of rice plants) 3（Tanaka）　　- Detection of PCR products by agarose gel electrophoresis 　　- Identification of a mutation lesson4: Plant genetics experiment (Genotyping of rice plants) 4（Tanaka）　　- Summary of Parts 1 to 3 　　- Writing a report lesson 5: Subcloning 1 (Fujikawa) 　　- Plasmid extraction from Escherichia coli lesson 6: Subcloning 2 (Fujikawa) 　　- Cutting and recombination of plasmids 　　-Confirmation of extracted plasmids lesson 7: Subcloning 3 (Fujikawa) 　　- Confirmation of cut plasmids 　　-Transformation of Escherichia coli lesson 8: Subcloning 4 (Fujikawa) 　　- Observation of colonies 　　-Report writing from Session 5 to Session 8 lesson9: Plant ecophysiology experiment 1（Tominaga） - Measuremetns of photosynthesis in leaves in laboratories lesson10: Plant ecophysiology experiment 2（Tominaga）　　- Measuremetns of photosynthesis in leaves in fields lesson11: Plant ecophysiology experiment 3（Tominaga）　　- Measuremetns of photosynthesis in leaves in fields lesson12: Plant ecophysiology experiment 4（Tominaga）　　- Summary of Parts 1 to 3 　　- Writing a report Reports are assigned according to the instructor in charge of the practical. After each experiment conducted by each instructor, submit an experiment report according to the instructions of the instructor. Lessions 1-4: Tanaka Lessions 5-8: Fujikawa Lessions 9-12: Tominaga The above schedule is tentative. Changes may occur depending on the progress of the experiment, etc.
Text/Reference Books,etc.	Textbook: A textbook for experiment will be given. Reference books: Bio-Experiment Illustrated 1: Basics of Molecular Biology Experiments (Cellular Engineering Supplement, Shujunsha) Experimental protocols for model plants: Rice, Arabidopsis, and Miyakogusa (Cellular Engineering Supplement, Shujunsha) Measurement and modelling of photosynthesis and production in plants (Kyouritsu shuppan)
PC or AV used in Class,etc.
(More Details)	Experimental equipments. PC.
Learning techniques to be incorporated
Suggestions on Preparation and Review	Read the textbook for experiment. Organize experiment records after each experiment. Also, individually further analyze the discussed contents. Prepare for the next experiment based on the distributed text. For sessions 5 to 8, be sure to take a comprehension test before starting the 5th session.
Requirements
Grading Method	Report(60%) & attitude during experiment(40%). However, according to the university regulations, if more than one-third of the classes are absent without permission, the performance cannot be evaluated.
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message	Always prepare an experiment notebook and bring a PC. This practical will be conducted by three instructors (Tanaka, Fujikawa, Tominaga).
Other
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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