Hiroshima University Syllabus

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Academic Year 2024Year School/Graduate School School of Applied Biological Science
Lecture Code L5006004 Subject Classification Specialized Education
Subject Name 分子農学生命科学実験II
Subject Name
Subject Name in
Laboratory Work in Molecular Agro-life ScienceII
タナカ ワカナ,フジカワ ユキチ,トミナガ ジュン
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  Intensive
Days, Periods, and Classrooms (Int) Inte
Lesson Style Experiment Lesson Style
(More Details)
Molecular Biological experiments. 
Credits 1.0 Class Hours/Week   Language of Instruction J : Japanese
Course Level 2 : Undergraduate Low-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 01 : Agricultural Sciences
Eligible Students
Keywords DNA isolation, PCR, Electrophoretic detection, PCR extraction, Restriction Enzyme treatment, ligation reaction, transformation 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
(Applicable only to targeted subjects for undergraduate students)
Molecular Agro-Life Science Program
(Abilities and Skills)
・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population
Class Objectives
/Class Outline
The main aim of this class is to learn experimental techniques in molecular biology. 
Class Schedule lesson1: Plant genetics experiment (Genotyping of rice plants) 1(Tanaka)
  - DNA isolation from plant tissue
  - Leaf sampling
  - DNA  isolation by alcohol precipitation
lesson2: Plant genetics experiment (Genotyping of rice plants) 2(Tanaka)
  - Amplification of a target gene by PCR reaction
lesson3: Plant genetics experiment (Genotyping of rice plants) 3(Tanaka)
  - Detection of PCR products by agarose gel electrophoresis
  - Identification of a mutation
lesson4: Plant genetics experiment (Genotyping of rice plants) 4(Tanaka)
  - Summary of Parts 1 to 3
  - Writing a report
lesson 5: Subcloning 1 (Fujikawa)
  - Plasmid extraction from Escherichia coli
lesson 6: Subcloning 2 (Fujikawa)
  - Cutting and recombination of plasmids
  -Confirmation of extracted plasmids
lesson 7: Subcloning 3 (Fujikawa)
  - Confirmation of cut plasmids
  -Transformation of Escherichia coli
lesson 8: Subcloning 4 (Fujikawa)
  - Observation of colonies
  -Report writing from Session 5 to Session 8
lesson9: Plant ecophysiology experiment 1(Tominaga)
    - Measuremetns of photosynthesis in leaves in laboratories
lesson10: Plant ecophysiology experiment 2(Tominaga)
  - Measuremetns of photosynthesis in leaves in fields
lesson11: Plant ecophysiology experiment 3(Tominaga)
  - Measuremetns of photosynthesis in leaves in fields
lesson12:  Plant ecophysiology experiment 4(Tominaga)
  - Summary of Parts 1 to 3
  - Writing a report

Reports are assigned according to the instructor in charge of the practical.
After each experiment conducted by each instructor, submit an experiment report according to the instructions of the instructor.
Lessions 1-4: Tanaka
Lessions 5-8: Fujikawa
Lessions 9-12: Tominaga

The above schedule is tentative. Changes may occur depending on the progress of the experiment, etc. 
Textbook: A textbook for experiment will be given.
Reference books:
Bio-Experiment Illustrated 1: Basics of Molecular Biology Experiments (Cellular Engineering Supplement, Shujunsha)
Experimental protocols for model plants: Rice, Arabidopsis, and Miyakogusa (Cellular Engineering Supplement, Shujunsha)
Measurement and modelling of photosynthesis and  production in plants (Kyouritsu shuppan) 
PC or AV used in
(More Details) Experimental equipments. PC. 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Read the textbook for experiment.
Organize experiment records after each experiment.
Also, individually further analyze the discussed contents.
Prepare for the next experiment based on the distributed text.
For sessions 5 to 8, be sure to take a comprehension test before starting the 5th session. 
Grading Method Report(60%) &  attitude during experiment(40%).
However, according to the university regulations, if more than one-third of the classes are absent without permission, the performance cannot be evaluated. 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message Always prepare an experiment notebook and bring a PC.
This practical will be conducted by three instructors (Tanaka, Fujikawa, Tominaga). 
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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