Hiroshima University Syllabus

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Academic Year 2024Year School/Graduate School School of Applied Biological Science
Lecture Code L5004004 Subject Classification Specialized Education
Subject Name 分子農学生命科学実験I
Subject Name
Subject Name in
Laboratory Work in Molecular Agro-life Science I
オオムラ ヒサシ,サンボンギ ヨシヒロ,フジイ ソウタロウ
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  Intensive
Days, Periods, and Classrooms (Int) Inte
Lesson Style Experiment Lesson Style
(More Details)
Credits 1.0 Class Hours/Week   Language of Instruction J : Japanese
Course Level 3 : Undergraduate High-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 01 : Agricultural Sciences
Eligible Students 2nd year students of the Faculty of Applied Biological Science
Keywords Solvent partition, organic synthesis, instrumental analysis, protein engineering,  
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
(Applicable only to targeted subjects for undergraduate students)
Molecular Agro-Life Science Program
(Abilities and Skills)
・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population
Class Objectives
/Class Outline
To learn basic technologies on the biological chemistry
To learn basic instrumental analyses
To learn basis of protein engineering 
Class Schedule 1) Solvent partition of organic compounds
2) Analyses of organic compounds by IR and TLC
3) Analyses of organic compounds by GC and HPLC
4) Collection of recombinant cytochrome c
5) Quantification of cytochrome c by absorption spectrum measurement and Bradford method
6) Check of cytochrome c expression by SDS-PAGE
7) Crystallization of lysozyme and overview of X-ray diffraction
8) Structural analysis based on the electron density map of lysozyme
9) Determination of the lysozyme structure 
A textbook will be distributed through moodle system, etc. 
PC or AV used in
(More Details) Textbook, writing on the blackboard, etc
Learning techniques to be incorporated  
Suggestions on
Preparation and
Read the textbook.
Before attending the first lecture, students should watch the on-demand video in Moodle.
The 1st to 3rd lectures will be held in the room H302 in School of Integrated Arts and Sciences. Be sure to bring labware.
The 4th and 9th lectures will be held in the room A701 in School of Applied Biological Science.

Grading Method Experiments (~30%) and reports (~70%) 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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