| Academic Year |
2024Year |
School/Graduate School |
School of Applied Biological Science |
| Lecture Code |
L5004004 |
Subject Classification |
Specialized Education |
| Subject Name |
分子農学生命科学実験I |
Subject Name
（Katakana） |
ブンシノウガクセイメイカガクジッケンI |
Subject Name in
English |
Laboratory Work in Molecular Agro-life Science I |
| Instructor |
OOMURA HISASHI,SAMBONGI YOSHIHIRO,FUJII SOTARO |
Instructor
(Katakana) |
オオムラ ヒサシ,サンボンギ ヨシヒロ,フジイ ソウタロウ |
| Campus |
Higashi-Hiroshima |
Semester/Term |
2nd-Year, Second Semester, Intensive |
| Days, Periods, and Classrooms |
(Int) Inte |
| Lesson Style |
Experiment |
Lesson Style
(More Details) |
|
Experiment
|
| Credits |
1.0 |
Class Hours/Week |
|
Language of Instruction |
J
:
Japanese |
| Course Level |
3
:
Undergraduate High-Intermediate
|
| Course Area（Area） |
26
:
Biological and Life Sciences |
| Course Area（Discipline） |
01
:
Agricultural Sciences |
| Eligible Students |
2nd year students of the Faculty of Applied Biological Science |
| Keywords |
Solvent partition, organic synthesis, instrumental analysis, protein engineering, |
| Special Subject for Teacher Education |
|
Special Subject |
|
Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) | |
|---|
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) | Molecular Agro-Life Science Program
（Abilities and Skills）
・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population
|
|---|
Class Objectives
/Class Outline |
To learn basic technologies on the biological chemistry
To learn basic instrumental analyses
To learn basis of protein engineering |
| Class Schedule |
1) Solvent partition of organic compounds
2) Analyses of organic compounds by IR and TLC
3) Analyses of organic compounds by GC and HPLC
4) Collection of recombinant cytochrome c
5) Quantification of cytochrome c by absorption spectrum measurement and Bradford method
6) Check of cytochrome c expression by SDS-PAGE
7) Crystallization of lysozyme and overview of X-ray diffraction
8) Structural analysis based on the electron density map of lysozyme
9) Determination of the lysozyme structure |
Text/Reference
Books,etc. |
A textbook will be distributed through moodle system, etc. |
PC or AV used in
Class,etc. |
|
| (More Details) |
Textbook, writing on the blackboard, etc
|
| Learning techniques to be incorporated |
|
Suggestions on
Preparation and
Review |
Read the textbook.
Before attending the first lecture, students should watch the on-demand video in Moodle.
The 1st to 3rd lectures will be held in the room H302 in School of Integrated Arts and Sciences. Be sure to bring labware.
The 4th and 9th lectures will be held in the room A701 in School of Applied Biological Science.
|
| Requirements |
|
| Grading Method |
Experiments (~30%) and reports (~70%) |
| Practical Experience |
|
| Summary of Practical Experience and Class Contents based on it |
|
| Message |
|
| Other |
|
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. |