HL030000 Practice for Fundamental Biology III

Hiroshima University Syllabus

Japanese

Academic Year 2024Year School/Graduate School School of Science

Lecture Code HL030000 Subject Classification Specialized Education

Subject Name 生物科学基礎実験III

Subject Name
（Katakana）セイブツカガクキソジッケン３

Subject Name in
English Practice for Fundamental Biology III

Instructor SHIMAMURA MASAKI,TAKAHASHI HARUKO,SHIMADA HIROSHI,KIKUCHI YUTAKA,SHIMODE SAYUMI,HOSOBA KOSUKE,HANADA HIDEKI,NAKATSUBO KEIKO,MORIGUCHI KAZUKI,TAZAWA ICHIRO,SUZUKI ATSUSHI,NAKAJIMA KEISUKE,FUKAZAWA JUTAROU,YAMAGUCHI TOMIO

Instructor
(Katakana) シマムラ　マサキ,タカハシ　ハルコ,シマダ　ヒロシ,キクチ　ユタカ,シモデ　サユミ,ホソバ　コウスケ,ハナダ　ヒデキ,ナカツボ　ケイコ,モリグチ　カズキ,タザワ　イチロウ,スズキ　アツシ,ナカジマ　ケイスケ,フカザワ　ジュタロウ,ヤマグチ　トミオ

Campus Higashi-Hiroshima Semester/Term 3rd-Year,  First Semester,  First Semester

Days, Periods, and Classrooms (1st) Weds5-10,Fri5-10

Lesson Style Experiment Lesson Style
(More Details) 　

Experiments, Fieldworks, Use of chemicals

Credits 6.0 Class Hours/Week 　 Language of Instruction J : Japanese

Course Level 3 : Undergraduate High-Intermediate

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 03 : Basic Biology

Eligible Students 3rd-year students of the Department of Biological Science

Keywords Developmental biology, Plant physiology, Molecular biology, Plant systematics, Animal Phylogenetics, Enzyme biochemistry

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) Students get the ability to conduct the experiments in Developmental biology, Plant physiology, Molecular biology, Plant systematics, Animal Phylogenetics, Enzyme biochemistry based on the basic knowledge.
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) Biology
（Abilities and Skills）
・Based on basic knowledge which is already acquired, to obtain the following experimental skills in order to practice research: 1) Basic observation skills and skills to manage experiments. 2) Ability to record observed natural phenomena. 3) Ability to collect and assess data.

Class Objectives
/Class Outline Students will learn experimental procedures for developmental biology, plant physiology, animal physiology, plant systematics and cell biology as the basis of the study of biological sciences.

Class Schedule 1. General guidance

2-6. Developmental biology
(1) Observation of early development of echinoderm
(2) Observation of early development of amphibian
(3) Analysis of expression patterns of morphogenetic genes during development

7-11. Plant physiology
(1) Control of germination by several plant hormones
(2) Transient assay using protoplast of Arabidopsis cultured cell
(3) The effect of plant hormone to plant growth and development
(4) Feedback regulation of plant hormone

12-16. Molecular biology
(1) Guidance for the experimental schedule, preparation of media and reagents, and construction of a recombinant plasmid
(2) Transformation of E. coli cells by introduction of the recombinant plasmid
(3) Analysis of the recombinant plasmid (1)
(4) Analysis of the recombinant plasmid (2)
(5) Expression analysis of a gene in the recombinant plasmid, Discussion

17-21. Plant  Phylogenetics
(1) Algae (Sea weeds)
(2) Charophycean algae
(3) Bryophytes
(4) Ferns and fern allies
(5) Seed plants

22-26. Animal Phylogenetics
(1) DNA extraction from animal tissue and PCR
(2) Purification of PCR product
(3) DNA sequencing
(4) Phylogenetic tree reconstruction based on molecular data -1
(5) Phylogenetic tree reconstruction based on molecular data -2

27-30. Neuroscience and Behavior genetics

Text/Reference
Books,etc. Textbook for "Practice for Fundamental Biology"
Some reference materials may be distributed for each experiment.

PC or AV used in
Class,etc. 　

(More Details) Textbook, PC, research equipments

Learning techniques to be incorporated 　

Suggestions on
Preparation and
Review Textbook for "Practice for Fundamental Biology" should be read before and after each experiment.
Report of each experiment should be prepared immediately after experiment.
Each report should include (1) purpose, (2) experimental procedures, (3) results and (4) discussions.

Requirements Students have to attend all experiments and any truancy is not allowed.

Grading Method The grade will be evaluated by reports (80%) and attitude (20%).

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message Because each experiment may involve the use of hazardous materials, students need to pay enough attention to the experimental procedures.

Other

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2024Year	School/Graduate School	School of Science
Lecture Code	HL030000	Subject Classification	Specialized Education
Subject Name	生物科学基礎実験III
Subject Name （Katakana）	セイブツカガクキソジッケン３
Subject Name in English	Practice for Fundamental Biology III
Instructor	SHIMAMURA MASAKI,TAKAHASHI HARUKO,SHIMADA HIROSHI,KIKUCHI YUTAKA,SHIMODE SAYUMI,HOSOBA KOSUKE,HANADA HIDEKI,NAKATSUBO KEIKO,MORIGUCHI KAZUKI,TAZAWA ICHIRO,SUZUKI ATSUSHI,NAKAJIMA KEISUKE,FUKAZAWA JUTAROU,YAMAGUCHI TOMIO
Instructor (Katakana)	シマムラ　マサキ,タカハシ　ハルコ,シマダ　ヒロシ,キクチ　ユタカ,シモデ　サユミ,ホソバ　コウスケ,ハナダ　ヒデキ,ナカツボ　ケイコ,モリグチ　カズキ,タザワ　イチロウ,スズキ　アツシ,ナカジマ　ケイスケ,フカザワ　ジュタロウ,ヤマグチ　トミオ
Campus	Higashi-Hiroshima	Semester/Term	3rd-Year, First Semester, First Semester
Days, Periods, and Classrooms	(1st) Weds5-10,Fri5-10
Lesson Style	Experiment	Lesson Style (More Details)
Experiments, Fieldworks, Use of chemicals
Credits	6.0	Class Hours/Week		Language of Instruction	J : Japanese
Course Level	3 : Undergraduate High-Intermediate
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	03 : Basic Biology
Eligible Students	3rd-year students of the Department of Biological Science
Keywords	Developmental biology, Plant physiology, Molecular biology, Plant systematics, Animal Phylogenetics, Enzyme biochemistry
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)	Students get the ability to conduct the experiments in Developmental biology, Plant physiology, Molecular biology, Plant systematics, Animal Phylogenetics, Enzyme biochemistry based on the basic knowledge.
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Biology （Abilities and Skills）・Based on basic knowledge which is already acquired, to obtain the following experimental skills in order to practice research: 1) Basic observation skills and skills to manage experiments. 2) Ability to record observed natural phenomena. 3) Ability to collect and assess data.
Class Objectives /Class Outline	Students will learn experimental procedures for developmental biology, plant physiology, animal physiology, plant systematics and cell biology as the basis of the study of biological sciences.
Class Schedule	1. General guidance 2-6. Developmental biology (1) Observation of early development of echinoderm (2) Observation of early development of amphibian (3) Analysis of expression patterns of morphogenetic genes during development 7-11. Plant physiology (1) Control of germination by several plant hormones (2) Transient assay using protoplast of Arabidopsis cultured cell (3) The effect of plant hormone to plant growth and development (4) Feedback regulation of plant hormone 12-16. Molecular biology (1) Guidance for the experimental schedule, preparation of media and reagents, and construction of a recombinant plasmid (2) Transformation of E. coli cells by introduction of the recombinant plasmid (3) Analysis of the recombinant plasmid (1) (4) Analysis of the recombinant plasmid (2) (5) Expression analysis of a gene in the recombinant plasmid, Discussion 17-21. Plant Phylogenetics (1) Algae (Sea weeds) (2) Charophycean algae (3) Bryophytes (4) Ferns and fern allies (5) Seed plants 22-26. Animal Phylogenetics (1) DNA extraction from animal tissue and PCR (2) Purification of PCR product (3) DNA sequencing (4) Phylogenetic tree reconstruction based on molecular data -1 (5) Phylogenetic tree reconstruction based on molecular data -2 27-30. Neuroscience and Behavior genetics
Text/Reference Books,etc.	Textbook for "Practice for Fundamental Biology" Some reference materials may be distributed for each experiment.
PC or AV used in Class,etc.
(More Details)	Textbook, PC, research equipments
Learning techniques to be incorporated
Suggestions on Preparation and Review	Textbook for "Practice for Fundamental Biology" should be read before and after each experiment. Report of each experiment should be prepared immediately after experiment. Each report should include (1) purpose, (2) experimental procedures, (3) results and (4) discussions.
Requirements	Students have to attend all experiments and any truancy is not allowed.
Grading Method	The grade will be evaluated by reports (80%) and attitude (20%).
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message	Because each experiment may involve the use of hazardous materials, students need to pay enough attention to the experimental procedures.
Other
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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