Academic Year |
2024Year |
School/Graduate School |
School of Integrated Arts and Sciences Department of Integrated Arts and Sciences |
Lecture Code |
ANL13001 |
Subject Classification |
Specialized Education |
Subject Name |
生命科学実験法B |
Subject Name (Katakana) |
セイメイカガクジッケンホウB |
Subject Name in English |
Practical Methods in Life Sciences B |
Instructor |
WASAKI JUN,KUGA YUKARI,NAKAGAWA NAOKI,KATSUYAMA CHIE |
Instructor (Katakana) |
ワサキ ジュン,クガ ユカリ,ナカガワ ナオキ,カツヤマ チエ |
Campus |
Higashi-Hiroshima |
Semester/Term |
2nd-Year, Second Semester, 4Term |
Days, Periods, and Classrooms |
(4T) Thur5,Fri5:IAS B414 |
Lesson Style |
Lecture |
Lesson Style (More Details) |
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Practice several experiments explained in Practical Methods in Life Sciences B |
Credits |
1.0 |
Class Hours/Week |
|
Language of Instruction |
J
:
Japanese |
Course Level |
3
:
Undergraduate High-Intermediate
|
Course Area(Area) |
26
:
Biological and Life Sciences |
Course Area(Discipline) |
04
:
Life Sciences |
Eligible Students |
|
Keywords |
Mycorrhiza, Symbiosis, Microbial culturing (bacteria & fungi), Sterilization Techniques, Staining, Microscopy, Multiple comparison analysis, Plant, Environmental Response, Nutrition, Rhizosphere, Enzyme, SDG_03, SDG_15 |
Special Subject for Teacher Education |
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Special Subject |
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Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students) | |
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Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students) | Integrated Arts and Sciences (Knowledge and Understanding) ・Knowledge and understanding of the importance and characteristics of each discipline and basic theoretical framework. (Abilities and Skills) ・The ability and skills to specify necessary theories and methods for consideration of issues. (Comprehensive Abilities) ・The ability to conduct research proactively by combining knowledge, understanding, and skills for the tasks, based on flexible creativity and imagination. |
Class Objectives /Class Outline |
To understand environmental microbiology, plant physiology and basic statistics, students practice selected experiments. |
Class Schedule |
lesson1 Environmental microbiology lab using mycorrhiza 1: Sterilization techniques, agar culture media formation (YK, CK) lesson2 Environmental microbiology lab using mycorrhiza 2: Mycorrhizal root sampling and fixation (YK, CK) lesson3 Environmental microbiology lab using mycorrhiza 3: Isolation of bacteria and fungi from the roots, rRNA gene amplification (YK, CK) lesson4 Environmental microbiology lab using mycorrhiza 4: Staining roots, preparation of slides (YK, CK) lesson5 Environmental microbiology lab using mycorrhiza 5: Observation of mycorrhiza, colonization rate measurements(YK, CK) lesson6 Environmental microbiology lab using mycorrhiza 6: Observation of isolated microbes, measurements of isolation rates (YK, CK) lesson7 Environmental microbiology lab using mycorrhiza 7: Multiple comparison analysis using R, electrophoresis of rRNA gene (YK, CK) lesson8 Laboratory work in plant physiology 1: Observation of plants grown under poor nutrient stresses (JW, NN) lesson9 Laboratory work in plant physiology 2: Growth analysis, digestion, and enzyme activity (JW, NN) lesson10 Laboratory work in plant physiology 3: Measurements of phosphorus content of plants (JW, NN) lesson11 Laboratory work in plant physiology 4:Measurements of nitrogen content of plants (JW, NN) lesson12 Laboratory work in plant physiology 5: Electrophoresis of protein (JW, NN) lesson13 Laboratory work in plant physiology 6: Quantification of mRNA for stress responsible genes (JW, NN) lesson14 Laboratory work in plant physiology 7: Analysis of results (JW, NN) lesson15 Summary |
Text/Reference Books,etc. |
Lecture notes for "Practical Methods in Life Sciences B". |
PC or AV used in Class,etc. |
|
(More Details) |
Experimental equipments for physiological measurements and bioinfomatic analysis. |
Learning techniques to be incorporated |
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Suggestions on Preparation and Review |
Read lecture notes carefully. |
Requirements |
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Grading Method |
Grade is judged by practice of the experiments, short talk on the results, and printed reports. |
Practical Experience |
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Summary of Practical Experience and Class Contents based on it |
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Message |
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Other |
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Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching. |