Hiroshima University Syllabus

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Japanese
Academic Year 2024Year School/Graduate School School of Integrated Arts and Sciences Department of Integrated Arts and Sciences
Lecture Code ANL13001 Subject Classification Specialized Education
Subject Name 生命科学実験法B
Subject Name
(Katakana)
セイメイカガクジッケンホウB
Subject Name in
English
Practical Methods in Life Sciences B
Instructor WASAKI JUN,KUGA YUKARI,NAKAGAWA NAOKI,KATSUYAMA CHIE
Instructor
(Katakana)
ワサキ ジュン,クガ ユカリ,ナカガワ ナオキ,カツヤマ チエ
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  4Term
Days, Periods, and Classrooms (4T) Thur5,Fri5:IAS B414
Lesson Style Lecture Lesson Style
(More Details)
 
Practice several experiments explained in Practical Methods in Life Sciences B 
Credits 1.0 Class Hours/Week   Language of Instruction J : Japanese
Course Level 3 : Undergraduate High-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 04 : Life Sciences
Eligible Students
Keywords Mycorrhiza, Symbiosis, Microbial culturing (bacteria & fungi), Sterilization Techniques, Staining, Microscopy, Multiple comparison analysis, Plant, Environmental Response, Nutrition, Rhizosphere, Enzyme, SDG_03, SDG_15 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students)
 
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students)
Integrated Arts and Sciences
(Knowledge and Understanding)
・Knowledge and understanding of the importance and characteristics of each discipline and basic theoretical framework.
(Abilities and Skills)
・The ability and skills to specify necessary theories and methods for consideration of issues.
(Comprehensive Abilities)
・The ability to conduct research proactively by combining knowledge,  understanding, and skills for the tasks, based on flexible creativity and imagination. 
Class Objectives
/Class Outline
To understand environmental microbiology, plant physiology and basic statistics, students practice selected experiments. 
Class Schedule lesson1 Environmental microbiology lab using mycorrhiza 1: Sterilization techniques, agar culture media formation (YK, CK)
lesson2 Environmental microbiology lab using mycorrhiza 2: Mycorrhizal root sampling and fixation (YK, CK)
lesson3 Environmental microbiology lab using mycorrhiza 3: Isolation of bacteria and fungi from the roots, rRNA gene amplification (YK, CK)
lesson4 Environmental microbiology lab using mycorrhiza 4: Staining roots, preparation of slides (YK, CK)
lesson5 Environmental microbiology lab using mycorrhiza 5: Observation of mycorrhiza, colonization rate measurements(YK, CK)
lesson6 Environmental microbiology lab using mycorrhiza 6: Observation of isolated microbes, measurements of isolation rates (YK, CK)
lesson7 Environmental microbiology lab using mycorrhiza 7: Multiple comparison analysis using R, electrophoresis of rRNA gene (YK, CK)
lesson8 Laboratory work in plant physiology 1: Observation of plants grown under poor nutrient stresses (JW, NN)
lesson9 Laboratory work in plant physiology 2: Growth analysis, digestion, and enzyme activity (JW, NN)
lesson10 Laboratory work in plant physiology 3: Measurements of phosphorus content of plants (JW, NN)
lesson11 Laboratory work in plant physiology 4:Measurements of nitrogen content of plants (JW, NN)
lesson12 Laboratory work in plant physiology 5: Electrophoresis of protein (JW, NN)
lesson13 Laboratory work in plant physiology 6:  Quantification of mRNA for stress responsible genes (JW, NN)
lesson14 Laboratory work in plant physiology 7: Analysis of results (JW, NN)
lesson15 Summary 
Text/Reference
Books,etc.
Lecture notes for "Practical Methods in Life Sciences B". 
PC or AV used in
Class,etc.
 
(More Details) Experimental equipments for physiological measurements and bioinfomatic analysis. 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Review
Read lecture notes carefully. 
Requirements  
Grading Method Grade is judged by practice of the experiments, short talk on the results, and printed reports. 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message  
Other   
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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