ANL12001 Laboratory Work in Life Sciences B

Hiroshima University Syllabus

Japanese

Academic Year 2024Year School/Graduate School School of Integrated Arts and Sciences Department of Integrated Arts and Sciences

Lecture Code ANL12001 Subject Classification Specialized Education

Subject Name 生命科学実験Ｂ

Subject Name
（Katakana）セイメイカガクジッケンB

Subject Name in
English Laboratory Work in Life Sciences B

Instructor WASAKI JUN,KUGA YUKARI,NAKAGAWA NAOKI,KATSUYAMA CHIE

Instructor
(Katakana) ワサキ　ジュン,クガ　ユカリ,ナカガワ　ナオキ,カツヤマ　チエ

Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  4Term

Days, Periods, and Classrooms (4T) Thur6-8,Fri6-8：IAS B414

Lesson Style Experiment Lesson Style
(More Details) 　

Practice several experiments explained in Practical Methods in Life Sciences B

Credits 1.0 Class Hours/Week 　 Language of Instruction J : Japanese

Course Level 3 : Undergraduate High-Intermediate

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 04 : Life Sciences

Eligible Students

Keywords Mycorrhiza, Symbiosis, Microbial culturing (bacteria & fungi), Sterilization Techniques, Staining, Microscopy, Multiple comparison analysis, Plant, Environmental Response, Nutrition, Rhizosphere, Enzyme, SDG_03, SDG_15

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) Integrated Arts and Sciences
（Knowledge and Understanding）
・Knowledge and understanding of the importance and characteristics of each discipline and basic theoretical framework.
（Abilities and Skills）
・The ability and skills to specify necessary theories and methods for consideration of issues.
・The abilities and skills to summarize one's own research in reports or academic papers, and to deliver presentations at a seminar or research meetings, and to answer questions.
（Comprehensive Abilities）
・The ability to conduct research proactively by combining knowledge,  understanding, and skills for the tasks, based on flexible creativity and imagination.

Class Objectives
/Class Outline To understand environmental microbiology, plant physiology and basic statistics, students practice selected experiments.

Class Schedule lesson1 Environmental microbiology lab using mycorrhiza 1: Sterilization techniques, agar culture media formation (YK, CK)
lesson2 Environmental microbiology lab using mycorrhiza 2: Mycorrhizal root sampling and fixation (YK, CK)
lesson3 Environmental microbiology lab using mycorrhiza 2: Isolation of bacteria and fungi from the roots, rRNA gene amplification (YK, CK)
lesson4 Environmental microbiology lab using mycorrhiza 3: Staining roots, preparation of slides (YK, CK)
lesson5 Environmental microbiology lab using mycorrhiza 4: Observation of mycorrhiza, colonization rate measurements(YK, CK)
lesson6 Environmental microbiology lab using mycorrhiza 6: Observation of isolated microbes, measurements of isolation rates (YK, CK)
lesson7 Environmental microbiology lab using mycorrhiza 7: Multiple comparison analysis using R, electrophoresis of rRNA gene (YK, CK)
lesson8  Laboratory work in plant physiology 1: Observation of plants grown under poor nutrient stresses (JW, NN)
lesson9  Laboratory work in plant physiology 2: Growth analysis, digestion, and enzyme activity (JW, NN)
lesson10 Laboratory work in plant physiology 3: Measurements of phosphorus content of plants (JW, NN)
lesson11 Laboratory work in plant physiology 4:Measurements of nitrogen content of plants (JW, NN)
lesson12 Laboratory work in plant physiology 5: Electrophoresis of protein (JW, NN)
lesson13 Laboratory work in plant physiology 6:  Quantification of mRNA for stress responsible genes (JW, NN)
lesson14 Laboratory work in plant physiology 7: Analysis of results (JW, NN)
lesson15 Summary

Text/Reference
Books,etc. Lecture notes for "Practical Methods in Life Sciences B".

PC or AV used in
Class,etc. 　

(More Details) Experimental equipments for physiological measurements and bioinfomatic analysis.

Learning techniques to be incorporated 　

Suggestions on
Preparation and
Review Read lecture notes carefully.

Requirements

Grading Method Grade is judged by practice of the experiments, short talk on the results, and printed reports.

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message

Other

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2024Year	School/Graduate School	School of Integrated Arts and Sciences Department of Integrated Arts and Sciences
Lecture Code	ANL12001	Subject Classification	Specialized Education
Subject Name	生命科学実験Ｂ
Subject Name （Katakana）	セイメイカガクジッケンB
Subject Name in English	Laboratory Work in Life Sciences B
Instructor	WASAKI JUN,KUGA YUKARI,NAKAGAWA NAOKI,KATSUYAMA CHIE
Instructor (Katakana)	ワサキ　ジュン,クガ　ユカリ,ナカガワ　ナオキ,カツヤマ　チエ
Campus	Higashi-Hiroshima	Semester/Term	2nd-Year, Second Semester, 4Term
Days, Periods, and Classrooms	(4T) Thur6-8,Fri6-8：IAS B414
Lesson Style	Experiment	Lesson Style (More Details)
Practice several experiments explained in Practical Methods in Life Sciences B
Credits	1.0	Class Hours/Week		Language of Instruction	J : Japanese
Course Level	3 : Undergraduate High-Intermediate
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	04 : Life Sciences
Eligible Students
Keywords	Mycorrhiza, Symbiosis, Microbial culturing (bacteria & fungi), Sterilization Techniques, Staining, Microscopy, Multiple comparison analysis, Plant, Environmental Response, Nutrition, Rhizosphere, Enzyme, SDG_03, SDG_15
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Integrated Arts and Sciences （Knowledge and Understanding）・Knowledge and understanding of the importance and characteristics of each discipline and basic theoretical framework. （Abilities and Skills）・The ability and skills to specify necessary theories and methods for consideration of issues. ・The abilities and skills to summarize one's own research in reports or academic papers, and to deliver presentations at a seminar or research meetings, and to answer questions. （Comprehensive Abilities）・The ability to conduct research proactively by combining knowledge, understanding, and skills for the tasks, based on flexible creativity and imagination.
Class Objectives /Class Outline	To understand environmental microbiology, plant physiology and basic statistics, students practice selected experiments.
Class Schedule	lesson1 Environmental microbiology lab using mycorrhiza 1: Sterilization techniques, agar culture media formation (YK, CK) lesson2 Environmental microbiology lab using mycorrhiza 2: Mycorrhizal root sampling and fixation (YK, CK) lesson3 Environmental microbiology lab using mycorrhiza 2: Isolation of bacteria and fungi from the roots, rRNA gene amplification (YK, CK) lesson4 Environmental microbiology lab using mycorrhiza 3: Staining roots, preparation of slides (YK, CK) lesson5 Environmental microbiology lab using mycorrhiza 4: Observation of mycorrhiza, colonization rate measurements(YK, CK) lesson6 Environmental microbiology lab using mycorrhiza 6: Observation of isolated microbes, measurements of isolation rates (YK, CK) lesson7 Environmental microbiology lab using mycorrhiza 7: Multiple comparison analysis using R, electrophoresis of rRNA gene (YK, CK) lesson8 Laboratory work in plant physiology 1: Observation of plants grown under poor nutrient stresses (JW, NN) lesson9 Laboratory work in plant physiology 2: Growth analysis, digestion, and enzyme activity (JW, NN) lesson10 Laboratory work in plant physiology 3: Measurements of phosphorus content of plants (JW, NN) lesson11 Laboratory work in plant physiology 4:Measurements of nitrogen content of plants (JW, NN) lesson12 Laboratory work in plant physiology 5: Electrophoresis of protein (JW, NN) lesson13 Laboratory work in plant physiology 6: Quantification of mRNA for stress responsible genes (JW, NN) lesson14 Laboratory work in plant physiology 7: Analysis of results (JW, NN) lesson15 Summary
Text/Reference Books,etc.	Lecture notes for "Practical Methods in Life Sciences B".
PC or AV used in Class,etc.
(More Details)	Experimental equipments for physiological measurements and bioinfomatic analysis.
Learning techniques to be incorporated
Suggestions on Preparation and Review	Read lecture notes carefully.
Requirements
Grading Method	Grade is judged by practice of the experiments, short talk on the results, and printed reports.
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message
Other
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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