Hiroshima University Syllabus

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Academic Year 2024Year School/Graduate School School of Integrated Arts and Sciences Department of Integrated Arts and Sciences
Lecture Code ANL10001 Subject Classification Specialized Education
Subject Name 生命科学実験法A
Subject Name
Subject Name in
Practical Methods in Life Sciences A
ウケナ カズヨシ,ネヒラ タツオ
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  3Term
Days, Periods, and Classrooms (3T) Thur5,Fri5:IAS B414
Lesson Style Lecture Lesson Style
(More Details)
Lecture is given using slides. 
Credits 1.0 Class Hours/Week   Language of Instruction J : Japanese
Course Level 2 : Undergraduate Low-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 04 : Life Sciences
Eligible Students
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
(Applicable only to targeted subjects for undergraduate students)
Integrated Arts and Sciences
(Knowledge and Understanding)
・Knowledge and understanding of the importance and characteristics of each discipline and basic theoretical framework.
(Abilities and Skills)
・The ability and skills to specify necessary theories and methods for consideration of issues.
(Comprehensive Abilities)
・The ability to conduct research proactively by combining knowledge,  understanding, and skills for the tasks, based on flexible creativity and imagination. 
Class Objectives
/Class Outline
Several methodologies for experiments with neuropeptides and organic molecules, which are required for "Laboratory Work in Life Sciences A", are explained. 
Class Schedule Lectures on experimental methods to be used in "practical Methods in Life Sciences A" are provided.
(1) Adjustment Mechanism of Blood Osmolarity
(2) Adjustment Mechanism of Blood Sugar
(3) Behavioral Observation under Hyperosmolarity
(4) Preparation of Tissue Section
(5) Immunohistochemical staining 1
(6) Immunohistochemical staining 2
(7) Observation of Morphology
(8) Discussion 1
(9) Double-Layer Distribution
(10) Extraction of Caffeine
(11) Synthesis of Aspirin
(12) Synthesis of Acetaminophen
(13) Enantiomer Separation
(14) Analysis of Commercially Available Drugs
(15) Discussion 2

1. Osmotic regulation
2. Blood glucose regulation
3. Morphological analysis of neuropeptides
4. Pharmacological analysis of behaviors
5. Isolation of a natural organic product
6. Syntheses of organic molecules
Lecture notes describing the methodologies of experiments are provided.  
PC or AV used in
(More Details) Slides 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Read the lecture notes carefully. 
Grading Method Grade is determined based on the results of "Laboratory Work in Life Sciences A". 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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