J1505040 Basic Technique for Microbiology and Immunology

Hiroshima University Syllabus

Japanese

Academic Year 2026Year School/Graduate School School of Dentistry Program of Dentistry

Lecture Code J1505040 Subject Classification Specialized Education

Subject Name 口腔微生物学・免疫学実習

Subject Name
（Katakana）コウクウビセイブツガク・メンエキガクジッシュウ

Subject Name in
English Basic Technique for Microbiology and Immunology

Instructor KOMATSUZAWA HITOSHI,ARIYOSHI WATARU,NAITO MARIKO,NISHIHAMA SAKI,TOBIUME KEI,MATSUO MIKI

Instructor
(Katakana) コマツザワ　ヒトシ,アリヨシ　ワタル,ナイトウ　マリコ,ニシハマ　サキ,トビウメ　ケイ,マツオ　ミキ

Campus Kasumi Semester/Term 3rd-Year,  First Semester,  First Semester

Days, Periods, and Classrooms (1T) Weds1-4：DEN Training Rm 1, (2T) Weds5-8：DEN Training Rm 1

Lesson Style Practical Lesson Style
(More Details) Face-to-face, Online (simultaneous interactive), Online (on-demand)

Lecture, Practice, Experiment, Presentation
IDC students (when not come to Japan): on demand

Credits 1.0 Class Hours/Week 4 Language of Instruction B : Japanese／English

Course Level 3 : Undergraduate High-Intermediate

Course Area（Area） 27 : Health Sciences

Course Area（Discipline） 02 : Dental Sciences

Eligible Students

Keywords [Bacteriology]
oral bacteria, dental caries, periodontitis, antibacterial agents, Bacterial staining method, pathogenic bacteria
[Mucosal Immunology]
innnate and adaptive immunity, mucosal immunity, lymphoid organs, thymus, spleen, Peyer's patch, lymphocytes, macrophage, flow cytometry

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) During lectures and practical training on this class, you can learn the details of research, such as infectious disease, drug resistance, host defense in our body and indigenous microbial flora, using experimental techniques required for infectious diseases. You can also learn basics regarding infectious diseases and host immune responses. Furthermore, participants learn basic research techniques, such as aseptic procedure, screenings of oral microorganisms, drug sensitivity tests, and common immunological methods for the analysis of the gut associated lymphoid tissues.
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) Dentistry
（Knowledge and Understanding）
・Knowledge regarding basic and clinical dental medicine
・Knowledge of life sciences
（Abilities and Skills）
・Basic skills required for conducting experiments and presentations
（Comprehensive Abilities）
・Rich humanity, understanding of professional responsibilities of a dentist, and general capabilities including basic knowledge, skills, and attitude required for putting such responsibilities into practice

Class Objectives
/Class Outline [Bacteriology]
① Understand the kinds and characteristics of oral bacteria
② Understand the antibacterial agents
③ Learn about aseptic operations
④ Learn about bacterial cultureing and staining methods
⑤ Understand oral infectious diseases and its relation with systemic diseases
[Mucosal Immunology]
①Deepening the understanding of the adaptive immune cells, and tissues.
②Learn basic experimental procedures for immunology.

Class Schedule Lecture 1 [Bacteriology:Lecture], April 8 (Wed), 8:45-12:00  Introduction、Gram staining
Lecture 2 [Bacteriology:Lecture], April 15 (Wed), 8:45-12:00
Gram staining, Hand-borne, droplet bacteria
Lecture 3 [Bacteriology:Lecture], April 22 (Wed), 8:45-12:00
Hand-borne, droplet bacteria
Lecture 4 [Bacteriology:practice], May 1 (Wed), 8:45-12:00
　　　　　Streptococci （10.25-Special lecture)
Lecture 5 [Bacteriology:practice], May 13 (Wed), 8:45-12:00
Streptococci
　　　　　　　　　　　　　　　　　　　　
Lecture 6 [Bacteriology:practice], May 20 (Wed), 8:45-12:00
Periodontal bacteria
Lecture 7 [Bacteriology:practice], May 27 (Wed), 8:45-12:00
periodontal bacteria, antibiotic susceptibility test
Lecture 8 [Bacteriology:practice], June 3 (Wed), 12:50-16:05
antibiotic susceptibility test

Lecture 9 [Bacteriology:practice], June 10 (Wed), 12:50-16:05
Review of the practice,（10.25-Special lecture)
Lecture 10 [Bacteriology:practice], June 17 (Wed), 12:50-16:05
Practical exam
Lecture 11 [Bacteriology:practice], June 24 (Wed), 12:50-16:05
Writing exam
Lecture 12 [Immunology: introduction], July 1 (Wed), 12：50-16：05
Students are required to bring their own laptops.

Lecture 13 [Immunology: practice], July 8 (Wed), 12：50-17：00
Group A: Isolation of lymphoid tissue from Mice.
Group B: Read the provided materials and complete the learning task.
Lecture 14 [Immunology: practice], July 15 (Wed), 12：50-17：00
Group B: Isolation of lymphoid tissue from Mice.
Group A: Read the provided materials and complete the learning task.
Lecture 15 [Immunology: review], July 22 (Wed), 12：50-16：05

Assessment for the immunology practical will be conducted through a written report.

Special lectures are subject to change.

During class 12 and 13, one of the two groups (A or B) will have the practical session. Group assignments will be announced on the Momiji board.

Text/Reference
Books,etc. Oral Microbiology and Immunology (ASM press)
Janeway's Immunobiology, 10th edition, Garland Science

PC or AV used in
Class,etc. Text, Handouts, moodle

(More Details) hand-out, movie streaming etc.

Learning techniques to be incorporated Discussions, Post-class Report

Suggestions on
Preparation and
Review Preparation and understanding before the laboratory practices is absolutely required for your efficient performance.

Requirements You have to have gotten credit regarding immunology for registration of this class.  If you are late or absent from class, tell your teacher by phone, e-mail, etc. You might fail to get credits if you are absent without permission. Bring and wear your white coat. Follow teacher's instructions.

Grading Method [Bacteriology] reports (10%), examination (90%), and your attitude.
[Immunology] reports (40%) and practice performance (60%)

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message

Other

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2026Year	School/Graduate School	School of Dentistry Program of Dentistry
Lecture Code	J1505040	Subject Classification	Specialized Education
Subject Name	口腔微生物学・免疫学実習
Subject Name （Katakana）	コウクウビセイブツガク・メンエキガクジッシュウ
Subject Name in English	Basic Technique for Microbiology and Immunology
Instructor	KOMATSUZAWA HITOSHI,ARIYOSHI WATARU,NAITO MARIKO,NISHIHAMA SAKI,TOBIUME KEI,MATSUO MIKI
Instructor (Katakana)	コマツザワ　ヒトシ,アリヨシ　ワタル,ナイトウ　マリコ,ニシハマ　サキ,トビウメ　ケイ,マツオ　ミキ
Campus	Kasumi	Semester/Term	3rd-Year, First Semester, First Semester
Days, Periods, and Classrooms	(1T) Weds1-4：DEN Training Rm 1, (2T) Weds5-8：DEN Training Rm 1
Lesson Style	Practical	Lesson Style (More Details)	Face-to-face, Online (simultaneous interactive), Online (on-demand)
Lecture, Practice, Experiment, Presentation IDC students (when not come to Japan): on demand
Credits	1.0	Class Hours/Week	4	Language of Instruction	B : Japanese／English
Course Level	3 : Undergraduate High-Intermediate
Course Area（Area）	27 : Health Sciences
Course Area（Discipline）	02 : Dental Sciences
Eligible Students
Keywords	[Bacteriology] oral bacteria, dental caries, periodontitis, antibacterial agents, Bacterial staining method, pathogenic bacteria [Mucosal Immunology] innnate and adaptive immunity, mucosal immunity, lymphoid organs, thymus, spleen, Peyer's patch, lymphocytes, macrophage, flow cytometry
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)	During lectures and practical training on this class, you can learn the details of research, such as infectious disease, drug resistance, host defense in our body and indigenous microbial flora, using experimental techniques required for infectious diseases. You can also learn basics regarding infectious diseases and host immune responses. Furthermore, participants learn basic research techniques, such as aseptic procedure, screenings of oral microorganisms, drug sensitivity tests, and common immunological methods for the analysis of the gut associated lymphoid tissues.
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Dentistry （Knowledge and Understanding）・Knowledge regarding basic and clinical dental medicine ・Knowledge of life sciences （Abilities and Skills）・Basic skills required for conducting experiments and presentations （Comprehensive Abilities）・Rich humanity, understanding of professional responsibilities of a dentist, and general capabilities including basic knowledge, skills, and attitude required for putting such responsibilities into practice
Class Objectives /Class Outline	[Bacteriology] ① Understand the kinds and characteristics of oral bacteria ② Understand the antibacterial agents ③ Learn about aseptic operations ④ Learn about bacterial cultureing and staining methods ⑤ Understand oral infectious diseases and its relation with systemic diseases [Mucosal Immunology] ①Deepening the understanding of the adaptive immune cells, and tissues. ②Learn basic experimental procedures for immunology.
Class Schedule	Lecture 1 [Bacteriology:Lecture], April 8 (Wed), 8:45-12:00 Introduction、Gram staining Lecture 2 [Bacteriology:Lecture], April 15 (Wed), 8:45-12:00 Gram staining, Hand-borne, droplet bacteria Lecture 3 [Bacteriology:Lecture], April 22 (Wed), 8:45-12:00 Hand-borne, droplet bacteria Lecture 4 [Bacteriology:practice], May 1 (Wed), 8:45-12:00 　　　　　Streptococci （10.25-Special lecture) Lecture 5 [Bacteriology:practice], May 13 (Wed), 8:45-12:00 Streptococci 　　　　　　　　　　　　　　　　　　　　 Lecture 6 [Bacteriology:practice], May 20 (Wed), 8:45-12:00 Periodontal bacteria Lecture 7 [Bacteriology:practice], May 27 (Wed), 8:45-12:00 periodontal bacteria, antibiotic susceptibility test Lecture 8 [Bacteriology:practice], June 3 (Wed), 12:50-16:05 antibiotic susceptibility test Lecture 9 [Bacteriology:practice], June 10 (Wed), 12:50-16:05 Review of the practice,（10.25-Special lecture) Lecture 10 [Bacteriology:practice], June 17 (Wed), 12:50-16:05 Practical exam Lecture 11 [Bacteriology:practice], June 24 (Wed), 12:50-16:05 Writing exam Lecture 12 [Immunology: introduction], July 1 (Wed), 12：50-16：05 Students are required to bring their own laptops. Lecture 13 [Immunology: practice], July 8 (Wed), 12：50-17：00 Group A: Isolation of lymphoid tissue from Mice. Group B: Read the provided materials and complete the learning task. Lecture 14 [Immunology: practice], July 15 (Wed), 12：50-17：00 Group B: Isolation of lymphoid tissue from Mice. Group A: Read the provided materials and complete the learning task. Lecture 15 [Immunology: review], July 22 (Wed), 12：50-16：05 Assessment for the immunology practical will be conducted through a written report. Special lectures are subject to change. During class 12 and 13, one of the two groups (A or B) will have the practical session. Group assignments will be announced on the Momiji board.
Text/Reference Books,etc.	Oral Microbiology and Immunology (ASM press) Janeway's Immunobiology, 10th edition, Garland Science
PC or AV used in Class,etc.	Text, Handouts, moodle
(More Details)	hand-out, movie streaming etc.
Learning techniques to be incorporated	Discussions, Post-class Report
Suggestions on Preparation and Review	Preparation and understanding before the laboratory practices is absolutely required for your efficient performance.
Requirements	You have to have gotten credit regarding immunology for registration of this class. If you are late or absent from class, tell your teacher by phone, e-mail, etc. You might fail to get credits if you are absent without permission. Bring and wear your white coat. Follow teacher's instructions.
Grading Method	[Bacteriology] reports (10%), examination (90%), and your attitude. [Immunology] reports (40%) and practice performance (60%)
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message
Other
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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