ANL12001 Laboratory Work in Life Sciences B

Hiroshima University Syllabus

Japanese

Academic Year 2026Year School/Graduate School School of Integrated Arts and Sciences Department of Integrated Arts and Sciences

Lecture Code ANL12001 Subject Classification Specialized Education

Subject Name 生命科学実験Ｂ

Subject Name
（Katakana）セイメイカガクジッケンB

Subject Name in
English Laboratory Work in Life Sciences B

Instructor WASAKI JUN,KUGA YUKARI,NAKAGAWA NAOKI,KATSUYAMA CHIE,YAMADA HIROTSUNA,UEKI TATSUYA

Instructor
(Katakana) ワサキ　ジュン,クガ　ユカリ,ナカガワ　ナオキ,カツヤマ　チエ,ヤマダ　ヒロツナ,ウエキ　タツヤ

Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  4Term

Days, Periods, and Classrooms (4T) Thur6-8,Fri6-8：IAS B414

Lesson Style Experiment Lesson Style
(More Details) Face-to-face

Practice several experiments explained in Practical Methods in Life Sciences B

Credits 1.0 Class Hours/Week 6 Language of Instruction J : Japanese

Course Level 3 : Undergraduate High-Intermediate

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 04 : Life Sciences

Eligible Students

Keywords Mycorrhiza, Symbiosis, Microbial culturing (bacteria & fungi), Sterilization Techniques, Staining, Microscopy, Multiple comparison analysis, Plant, Environmental response, Nutrition, Rhizosphere, Enzyme, Animal, Metal, Protein purification, SDG_03, SDG_15

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) Integrated Arts and Sciences
（Knowledge and Understanding）
・Knowledge and understanding of the importance and characteristics of each discipline and basic theoretical framework.
（Abilities and Skills）
・The ability and skills to specify necessary theories and methods for consideration of issues.
・The abilities and skills to summarize one's own research in reports or academic papers, and to deliver presentations at a seminar or research meetings, and to answer questions.
（Comprehensive Abilities）
・The ability to conduct research proactively by combining knowledge,  understanding, and skills for the tasks, based on flexible creativity and imagination.

Class Objectives
/Class Outline To understand environmental microbiology, plant physiology and basic statistics, students practice selected experiments.

Class Schedule lesson1 Environmental microbiology 1: Mycorrhizal root sampling and sterilized technique (YK, CK)
lesson2 Environmental microbiology 2: Staining roots, preparation of slides (YK, CK)
lesson3 Environmental microbiology 3: Observation of stained roots, colonization rate of mycorrhizal fungi, and statistics (YK, CK)
lesson4 Environmental microbiology 4: PCR and electrophoresis of microbial rRNA genes from roots (YK, CK)
lesson5 Environmental microbiology 5: Observation of cultivated endophytic microbes, isolation rates (YK, CK)
lesson6 Laboratory work in plant physiology 1: Observation of plants grown under poor nutrient stresses (JW, NN, HY)
lesson7 Laboratory work in plant physiology 2: Growth analysis, digestion, and enzyme activity (JW, NN, HY)
lesson8  Laboratory work in plant physiology 3: Measurements of phosphorus content of plants (JW, NN, HY)
lesson9  Laboratory work in plant physiology 4:Measurements of nitrogen content of plants (JW, NN, HY)
lesson10 Laboratory work in plant physiology 5: Electrophoresis of protein (JW, NN, HY)
lesson11 Animal Physiology Experiment 1: Animal Dissection, Heart Rate Measurement, and Blood Cell Collection (TU)
lesson12 Animal Physiology Experiment 2: Blood Cell Protein Extraction (TU)
lesson13 Animal Physiology Experiment 3: Affinity Purification of Proteins (TU)
lesson14 Animal Physiology Experiment 4: Protein Electrophoresis (TU)
lesson15 Animal Physiology Experiment 5: Data Analysis (TU)

Text/Reference
Books,etc. Lecture notes for "Practical Methods in Life Sciences B".

PC or AV used in
Class,etc. Handouts, Visual Materials

(More Details) Experimental equipments for physiological measurements and bioinfomatic analysis.

Learning techniques to be incorporated Discussions, Quizzes/ Quiz format, Fieldwork / Survey, Post-class Report

Suggestions on
Preparation and
Review Read lecture notes carefully.

Requirements

Grading Method Grade is judged by practice of the experiments, short talk on the results, and printed reports.

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message

Other

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2026Year	School/Graduate School	School of Integrated Arts and Sciences Department of Integrated Arts and Sciences
Lecture Code	ANL12001	Subject Classification	Specialized Education
Subject Name	生命科学実験Ｂ
Subject Name （Katakana）	セイメイカガクジッケンB
Subject Name in English	Laboratory Work in Life Sciences B
Instructor	WASAKI JUN,KUGA YUKARI,NAKAGAWA NAOKI,KATSUYAMA CHIE,YAMADA HIROTSUNA,UEKI TATSUYA
Instructor (Katakana)	ワサキ　ジュン,クガ　ユカリ,ナカガワ　ナオキ,カツヤマ　チエ,ヤマダ　ヒロツナ,ウエキ　タツヤ
Campus	Higashi-Hiroshima	Semester/Term	2nd-Year, Second Semester, 4Term
Days, Periods, and Classrooms	(4T) Thur6-8,Fri6-8：IAS B414
Lesson Style	Experiment	Lesson Style (More Details)	Face-to-face
Practice several experiments explained in Practical Methods in Life Sciences B
Credits	1.0	Class Hours/Week	6	Language of Instruction	J : Japanese
Course Level	3 : Undergraduate High-Intermediate
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	04 : Life Sciences
Eligible Students
Keywords	Mycorrhiza, Symbiosis, Microbial culturing (bacteria & fungi), Sterilization Techniques, Staining, Microscopy, Multiple comparison analysis, Plant, Environmental response, Nutrition, Rhizosphere, Enzyme, Animal, Metal, Protein purification, SDG_03, SDG_15
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Integrated Arts and Sciences （Knowledge and Understanding）・Knowledge and understanding of the importance and characteristics of each discipline and basic theoretical framework. （Abilities and Skills）・The ability and skills to specify necessary theories and methods for consideration of issues. ・The abilities and skills to summarize one's own research in reports or academic papers, and to deliver presentations at a seminar or research meetings, and to answer questions. （Comprehensive Abilities）・The ability to conduct research proactively by combining knowledge, understanding, and skills for the tasks, based on flexible creativity and imagination.
Class Objectives /Class Outline	To understand environmental microbiology, plant physiology and basic statistics, students practice selected experiments.
Class Schedule	lesson1 Environmental microbiology 1: Mycorrhizal root sampling and sterilized technique (YK, CK) lesson2 Environmental microbiology 2: Staining roots, preparation of slides (YK, CK) lesson3 Environmental microbiology 3: Observation of stained roots, colonization rate of mycorrhizal fungi, and statistics (YK, CK) lesson4 Environmental microbiology 4: PCR and electrophoresis of microbial rRNA genes from roots (YK, CK) lesson5 Environmental microbiology 5: Observation of cultivated endophytic microbes, isolation rates (YK, CK) lesson6 Laboratory work in plant physiology 1: Observation of plants grown under poor nutrient stresses (JW, NN, HY) lesson7 Laboratory work in plant physiology 2: Growth analysis, digestion, and enzyme activity (JW, NN, HY) lesson8 Laboratory work in plant physiology 3: Measurements of phosphorus content of plants (JW, NN, HY) lesson9 Laboratory work in plant physiology 4:Measurements of nitrogen content of plants (JW, NN, HY) lesson10 Laboratory work in plant physiology 5: Electrophoresis of protein (JW, NN, HY) lesson11 Animal Physiology Experiment 1: Animal Dissection, Heart Rate Measurement, and Blood Cell Collection (TU) lesson12 Animal Physiology Experiment 2: Blood Cell Protein Extraction (TU) lesson13 Animal Physiology Experiment 3: Affinity Purification of Proteins (TU) lesson14 Animal Physiology Experiment 4: Protein Electrophoresis (TU) lesson15 Animal Physiology Experiment 5: Data Analysis (TU)
Text/Reference Books,etc.	Lecture notes for "Practical Methods in Life Sciences B".
PC or AV used in Class,etc.	Handouts, Visual Materials
(More Details)	Experimental equipments for physiological measurements and bioinfomatic analysis.
Learning techniques to be incorporated	Discussions, Quizzes/ Quiz format, Fieldwork / Survey, Post-class Report
Suggestions on Preparation and Review	Read lecture notes carefully.
Requirements
Grading Method	Grade is judged by practice of the experiments, short talk on the results, and printed reports.
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message
Other
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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