| Academic Year |
2025Year |
School/Graduate School |
Graduate School of Integrated Sciences for Life (Master's Course) |
| Lecture Code |
WI101600 |
Subject Classification |
Specialized Education |
| Subject Name |
ゲノム編集基礎演習 |
Subject Name
（Katakana） |
ゲノムヘンシュウキソエンシュウ |
Subject Name in
English |
Basic Training of Genome Editing |
| Instructor |
HOSOBA KOSUKE,SHIMODE SAYUMI,SUGI TAKUMA,KUME KODAI,MIURA KENTO |
Instructor
(Katakana) |
ホソバ コウスケ,シモデ サユミ,スギ タクマ,クメ コウダイ,ミウラ ケント |
| Campus |
Higashi-Hiroshima |
Semester/Term |
1st-Year, First Semester, OutOfTerm(1st) |
| Days, Periods, and Classrooms |
(O1) Inte：See the timetable for detail. |
| Lesson Style |
Seminar |
Lesson Style
(More Details) |
Face-to-face, Online (simultaneous interactive), Online (on-demand) |
| Experiment |
| Credits |
2.0 |
Class Hours/Week |
|
Language of Instruction |
B
:
Japanese／English |
| Course Level |
5
:
Graduate Basic
|
| Course Area（Area） |
26
:
Biological and Life Sciences |
| Course Area（Discipline） |
04
:
Life Sciences |
| Eligible Students |
Students in the Frontier Development Program for Genome Editing |
| Keywords |
Genome editing |
| Special Subject for Teacher Education |
|
Special Subject |
|
Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) | |
|---|
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) | |
|---|
Class Objectives
/Class Outline |
Learn the basic technology of genome editing using cultured cells (how to handle human cultured cells, targeted mutagenesis using CRISPR-Cas9, and analytical methods of introduced mutations) |
| Class Schedule |
Human cell culture
Introduction of CRISPR-Cas9 system into human cells
Learning of designing guide RNA and searching potential off-target sit
Learning of designing gene knock-in and genotyping primers
Mutation analysis using heteroduplex mobility assay and Cel-I assay (on- and off-target analyses)
Report |
Text/Reference
Books,etc. |
The course text will be distributed. |
PC or AV used in
Class,etc. |
|
| (More Details) |
PowerPoint and the like |
| Learning techniques to be incorporated |
|
Suggestions on
Preparation and
Review |
Preparation and review are recommended according to the course text. |
| Requirements |
Personal computer is required for the course. |
| Grading Method |
A grading will be given by evaluating the attitude and report. |
| Practical Experience |
|
| Summary of Practical Experience and Class Contents based on it |
|
| Message |
This course will be conducted in Innovation Plaza of Hiroshima University. |
| Other |
|
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. |