WB003100 Life Science and Gene Technology B

Hiroshima University Syllabus

Japanese

Academic Year 2025Year School/Graduate School Graduate School of Integrated Sciences for Life (Master's Course) Division of Integrated Sciences for Life Program of Biotechnology

Lecture Code WB003100 Subject Classification Specialized Education

Subject Name 生命機能工学Ｂ

Subject Name
（Katakana）セイメイキノウコウガクB

Subject Name in
English Life Science and Gene Technology B

Instructor KITAMURA KENJI,FUNABASHI HISAKAGE,KATO SETSU,FUJII TATSUYA

Instructor
(Katakana) キタムラ　ケンジ,フナバシ　ヒサカゲ,カトウ　セツ,フジイ　タツヤ

Campus Higashi-Hiroshima Semester/Term 1st-Year, Second Semester, 3Term

Days, Periods, and Classrooms (3T) Inte：Online

Lesson Style Lecture Lesson Style
(More Details) Online (on-demand)

Lecture

Credits 2.0 Class Hours/Week 　 Language of Instruction B : Japanese／English

Course Level 5 : Graduate Basic

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 02 : Biotechnology

Eligible Students Master course students

Keywords Bio-device, Biofunctional materials, Biological battery (Biofuel cell), Genome modification, Recombinant DNA technology Genome editing, Gene drive, Compliance, ABS, Nagoya Protocol, Genetic resources, Synthetic biology, Amino acid, Oligopeptide, Carbon neutral (CN), Microbes, Enzymes, Biomass, Biomanufacturing, Single-cell observation
SDG_3, SDG_07, SDG_09, SDG_14, SDG_15

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students)

Class Objectives
/Class Outline [Objective] Living organisms carry out their life activities through various chemical reactions in their cells and, in the case of multicellular organisms, through cell-cell interactions. Although these are complex phenomena, scientific advances are deepening our understanding of the molecular basis of these complex phenomena. Biotechnology is an example of developing new technologies based on these results and using them to benefit human society. In this lecture, five faculty members will explain specific examples, research methods, and rules to follow.

[Outline]
1) Microbes and enzymes are used for various useful materials production, environmental detoxification, and so on. This lecture introduces the various functions of microbes and enzymes. The students are expected to become possible to understand the usefulness of microbes and enzymes.
2) The use of genetically modified organisms created by genetically modified technology has expanded beyond biology to a wide range of fields from medical to agriculture and industry, and is now an indispensable part of industry. On the other hand, since genetically modified organisms are artificially created organisms that do not exist in nature, some of them pose risks to humans and the environment. Therefore, how to use genetically modified organisms safely is an important issue for us. In this lecture, I will explain the concept and method of safe handling that should be taken to obtain the maximum benefit from genetically modified organisms, based on the underlying gene recombination technology and genome editing technology.
3) Explain Nagoya protocol and utilization of digital sequence information in synthetic biology for compliance in life science research. Explain amino acids/oligopeptides-related topics; physiological action, sensing and cellular control of their utilization, related subjects in medical research.
4) The lecture introduces biofunctional engineering and cellular engineering. Especially the technique creating biodevices that utilize biofunctional materials such as enzymes, nucleic acids and cells will be focused. The students are expected to become possible to discuss the basic ideas, techniques and the perspectives of biodevices made by biofunctional materials.
5) Microbial cells typically exist as populations in their natural environments. It is well known that even within the same clonal population, a single cell can exhibit various behaviors. Therefore, knowing how this kind of phenotypic diversity is generated makes it possible to control the function and behavior of microbe populations. Understanding phenotypic variation in microbial populations and mastering detection methods are the goals of this course.

Class Schedule lesson 1. Techniques to develop biodevices utilizing biofunctional molecules. I. (Funabashi)
lesson 2. Techniques to develop biodevices utilizing biofunctional molecules. II. (Funabashi)
lesson 3. Techniques to develop biodevices utilizing living cells. I. (Funabashi)
lesson 4. Techniques to develop biodevices utilizing living cells. II. (Funabashi)
lesson 5. What is the genetic modification technology？ (Tanaka)
lesson 6. How to regulate genetically recombined microorganisms – Asilomar Conference - (Tanaka)
lesson 7. How to manage genetically recombined organisms – Cartagena Protocol - (Tanaka)
lesson 8. Nagoya Protocol and ABS. (Kitamura)
lesson 9. Digital sequence information and synthetic biology. (Kitamura)
lesson 10. Physiological action of amino acids/oligopeptides. (Kitamura)
lesson 11. Control of biosynthesis and utilization of amino acids/oligopeptides. (Kitamura)
lesson 12. Amino acids and oligopeptides in medical research. (Kitamura)
lesson 13. Effective utilization of microbes and enzymes (Fujii)
lesson 14. What is phenotypic diversity? (Kato)
lesson 15. How to measure phenotypic diversity at a single cell level (Kato)

Quiz using Moodle after each lesson.
Final examination (final report)

Text/Reference
Books,etc. ゲノム編集入門　山本　卓編（裳華房）、完全版　ゲノム編集実験スタンダード（羊土社）、酵母菌・麹菌・乳酸菌の産業応用展開　五味勝也 , 阿部敬悦監修（シーエムシー出版）

PC or AV used in
Class,etc. Handouts, Visual Materials, Microsoft Teams, Microsoft Stream, moodle

(More Details) Handout (Power Point)

Learning techniques to be incorporated Quizzes/ Quiz format

Suggestions on
Preparation and
Review lesson 1 Understand the utilization of enzymes and biofunctional proteins as a material.
lesson 2 Understand the utilization of nucleic acids as a material.
lesson 3 Understand the utilization of living bacteria as a material.
lesson 4 Understand the utilization of living mammalian cells as a material.
lesson 5 Understand what genetic modification technology is.
lesson 6 Understand the concept of containment of genetically modified microorganisms.
lesson 7 Understand the regulations governing the movement of genetically modified organisms internationally and the handling of genome-edited organisms in Japan.
lesson 8 Understand the concepts of Nagoya protocol and ABS for utilization of oversea genetic materials in biochemical studies.
lesson 9 Understand the problem for utilizing gene sequence database information for genetic modification of living organisms in synthetic biology.
lesson 10 Understand the cellular function of amino acids/oligopeptides, and cellular transporters for their uptake.
lesson 11 Understand the cellular sensing mechanism of amino acids/oligopeptides, general control of biosynthetic pathway of amino acids and cellular function.
lesson 12 Understand the amino acid-related topics in medical research.
lesson 13 Understand the various functions of microbes and enzymes.
lesson 14 Understand the characteristics and functions of microbes at a single-cell level.
lesson 15 Understand the techniques to detect phenotypic diversity in a microbial population.

Requirements Nothing

Grading Method Up to 50 points for the term-end examination.
Up to 50 points for the quizze each time.

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message Nothing

Other Nothing

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2025Year	School/Graduate School	Graduate School of Integrated Sciences for Life (Master's Course) Division of Integrated Sciences for Life Program of Biotechnology
Lecture Code	WB003100	Subject Classification	Specialized Education
Subject Name	生命機能工学Ｂ
Subject Name （Katakana）	セイメイキノウコウガクB
Subject Name in English	Life Science and Gene Technology B
Instructor	KITAMURA KENJI,FUNABASHI HISAKAGE,KATO SETSU,FUJII TATSUYA
Instructor (Katakana)	キタムラ　ケンジ,フナバシ　ヒサカゲ,カトウ　セツ,フジイ　タツヤ
Campus	Higashi-Hiroshima	Semester/Term	1st-Year, Second Semester, 3Term
Days, Periods, and Classrooms	(3T) Inte：Online
Lesson Style	Lecture	Lesson Style (More Details)	Online (on-demand)
Lecture
Credits	2.0	Class Hours/Week		Language of Instruction	B : Japanese／English
Course Level	5 : Graduate Basic
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	02 : Biotechnology
Eligible Students	Master course students
Keywords	Bio-device, Biofunctional materials, Biological battery (Biofuel cell), Genome modification, Recombinant DNA technology Genome editing, Gene drive, Compliance, ABS, Nagoya Protocol, Genetic resources, Synthetic biology, Amino acid, Oligopeptide, Carbon neutral (CN), Microbes, Enzymes, Biomass, Biomanufacturing, Single-cell observation SDG_3, SDG_07, SDG_09, SDG_14, SDG_15
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)
Class Objectives /Class Outline	[Objective] Living organisms carry out their life activities through various chemical reactions in their cells and, in the case of multicellular organisms, through cell-cell interactions. Although these are complex phenomena, scientific advances are deepening our understanding of the molecular basis of these complex phenomena. Biotechnology is an example of developing new technologies based on these results and using them to benefit human society. In this lecture, five faculty members will explain specific examples, research methods, and rules to follow. [Outline] 1) Microbes and enzymes are used for various useful materials production, environmental detoxification, and so on. This lecture introduces the various functions of microbes and enzymes. The students are expected to become possible to understand the usefulness of microbes and enzymes. 2) The use of genetically modified organisms created by genetically modified technology has expanded beyond biology to a wide range of fields from medical to agriculture and industry, and is now an indispensable part of industry. On the other hand, since genetically modified organisms are artificially created organisms that do not exist in nature, some of them pose risks to humans and the environment. Therefore, how to use genetically modified organisms safely is an important issue for us. In this lecture, I will explain the concept and method of safe handling that should be taken to obtain the maximum benefit from genetically modified organisms, based on the underlying gene recombination technology and genome editing technology. 3) Explain Nagoya protocol and utilization of digital sequence information in synthetic biology for compliance in life science research. Explain amino acids/oligopeptides-related topics; physiological action, sensing and cellular control of their utilization, related subjects in medical research. 4) The lecture introduces biofunctional engineering and cellular engineering. Especially the technique creating biodevices that utilize biofunctional materials such as enzymes, nucleic acids and cells will be focused. The students are expected to become possible to discuss the basic ideas, techniques and the perspectives of biodevices made by biofunctional materials. 5) Microbial cells typically exist as populations in their natural environments. It is well known that even within the same clonal population, a single cell can exhibit various behaviors. Therefore, knowing how this kind of phenotypic diversity is generated makes it possible to control the function and behavior of microbe populations. Understanding phenotypic variation in microbial populations and mastering detection methods are the goals of this course.
Class Schedule	lesson 1. Techniques to develop biodevices utilizing biofunctional molecules. I. (Funabashi) lesson 2. Techniques to develop biodevices utilizing biofunctional molecules. II. (Funabashi) lesson 3. Techniques to develop biodevices utilizing living cells. I. (Funabashi) lesson 4. Techniques to develop biodevices utilizing living cells. II. (Funabashi) lesson 5. What is the genetic modification technology？ (Tanaka) lesson 6. How to regulate genetically recombined microorganisms – Asilomar Conference - (Tanaka) lesson 7. How to manage genetically recombined organisms – Cartagena Protocol - (Tanaka) lesson 8. Nagoya Protocol and ABS. (Kitamura) lesson 9. Digital sequence information and synthetic biology. (Kitamura) lesson 10. Physiological action of amino acids/oligopeptides. (Kitamura) lesson 11. Control of biosynthesis and utilization of amino acids/oligopeptides. (Kitamura) lesson 12. Amino acids and oligopeptides in medical research. (Kitamura) lesson 13. Effective utilization of microbes and enzymes (Fujii) lesson 14. What is phenotypic diversity? (Kato) lesson 15. How to measure phenotypic diversity at a single cell level (Kato) Quiz using Moodle after each lesson. Final examination (final report)
Text/Reference Books,etc.	ゲノム編集入門　山本　卓編（裳華房）、完全版　ゲノム編集実験スタンダード（羊土社）、酵母菌・麹菌・乳酸菌の産業応用展開　五味勝也 , 阿部敬悦監修（シーエムシー出版）
PC or AV used in Class,etc.	Handouts, Visual Materials, Microsoft Teams, Microsoft Stream, moodle
(More Details)	Handout (Power Point)
Learning techniques to be incorporated	Quizzes/ Quiz format
Suggestions on Preparation and Review	lesson 1 Understand the utilization of enzymes and biofunctional proteins as a material. lesson 2 Understand the utilization of nucleic acids as a material. lesson 3 Understand the utilization of living bacteria as a material. lesson 4 Understand the utilization of living mammalian cells as a material. lesson 5 Understand what genetic modification technology is. lesson 6 Understand the concept of containment of genetically modified microorganisms. lesson 7 Understand the regulations governing the movement of genetically modified organisms internationally and the handling of genome-edited organisms in Japan. lesson 8 Understand the concepts of Nagoya protocol and ABS for utilization of oversea genetic materials in biochemical studies. lesson 9 Understand the problem for utilizing gene sequence database information for genetic modification of living organisms in synthetic biology. lesson 10 Understand the cellular function of amino acids/oligopeptides, and cellular transporters for their uptake. lesson 11 Understand the cellular sensing mechanism of amino acids/oligopeptides, general control of biosynthetic pathway of amino acids and cellular function. lesson 12 Understand the amino acid-related topics in medical research. lesson 13 Understand the various functions of microbes and enzymes. lesson 14 Understand the characteristics and functions of microbes at a single-cell level. lesson 15 Understand the techniques to detect phenotypic diversity in a microbial population.
Requirements	Nothing
Grading Method	Up to 50 points for the term-end examination. Up to 50 points for the quizze each time.
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message	Nothing
Other	Nothing
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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