I7062002 Genetic Engineering

Hiroshima University Syllabus

Japanese

Academic Year 2025Year School/Graduate School School of Pharmaceutical Sciences Program of Pharmaceutical Sciences

Lecture Code I7062002 Subject Classification Specialized Education

Subject Name 遺伝子工学

Subject Name
（Katakana）イデンシコウガク

Subject Name in
English Genetic Engineering

Instructor KUMAGAI TAKANORI

Instructor
(Katakana) クマガイ　タカノリ

Campus Kasumi Semester/Term 3rd-Year, Second Semester, Intensive

Days, Periods, and Classrooms (Int) Inte：霞R404講義室

Lesson Style Lecture Lesson Style
(More Details) Face-to-face

Lecture

Credits 2.0 Class Hours/Week 　 Language of Instruction B : Japanese／English

Course Level 3 : Undergraduate High-Intermediate

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 04 : Life Sciences

Eligible Students

Keywords restriction enzyme, vector, host, cloning, library, hybridization, DNA sequence, polymerase chain reaction, mutagenesis, gene introduction, gene therapy, genome

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) The second step: Structure and function of organisms
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) Pharmaceutical Sciences
（Knowledge and Understanding）
・Knowledge and understanding of the biological maintenance system of homeostasis and the ability to adjust to the environment ●quality⑤

Medicinal Sciences
（Knowledge and Understanding）
・Knowledge of human and biological bodies.
（Abilities and Skills）
・Development of knowledge about human and biological bodies. (advance)
・To be able to basically treat major chemical agents, substances related to the living body, and microbes.

Class Objectives
/Class Outline Understand the basic of genetic manipulation and study applicatios of genetic engineering in pharmaceutical field

Class Schedule lesson 1
○Guidance
○History of genetic engineering
lesson 2
○Tools for genetic engineering (1)
　Restriction enzymes
　Modification enzymes
　Vectors
lesson 3
○Tools for genetic engineering (2)
　Hosts
○Cartagena protocol on Biosafety
lesson4
○Gene cloning (1)
　Cloning with plasmid vector
　Preparation of phage library
　Preparation of cosmid library
lesson 5
○Gene cloning (2)
　Preparation of cDNA library
○Analysis of gene expression (Northern analysis, Western analysis, etc.)
lesson 6
○Overproduction of proteins (1)
　E. coli expression system
lesson 7
○Summary (Lessons 1～6)
lesson 8
○Overproduction of proteins (2)
　Brevibacillus expression system
　Yeast expression system
　Baculovirus expression system
　Mammalian expression system
　Cell-free expression system
lesson 9
○Biopharmaceutical
lesson 10
○DNA sequencing
　Sanger sequencing
　Next generation sequencing
lesson 11
○Polymerase chain reaction (PCR) and its application (1)
　Principle of PCR
lesson 12
○PCR and its application (2)
　Application of PCR
lesson 13
○Mutagenesis to DNA
　Random mutagenesis
　Site-directed mutagenesis
○Introduction of DNA into mammalian cells and gene therapy (1)
　Introduction of DNA into mammalian cells
lesson 14
○Introduction of DNA into mammalian cells and gene therapy (2)
　Gene therapy
lesson 15
○Summary (Lessons 8～14)

Text/Reference
Books,etc. Bioscience of the gene and protein (Sankyo Shuppan)

PC or AV used in
Class,etc. Handouts, Other (see [More Details]), moodle

(More Details) Original prints, PowerPoint.
Video of lectures are uploaded on moodle (for review).

Learning techniques to be incorporated

Suggestions on
Preparation and
Review Understand well the following points
Lesson 1
Experiments leading to the development of genetic engineering
Lesson 2
Characteristics of enzymes and vectors used for genetic engineering
Lesson 3
Characteristics of hosts used for genetic engineering
Ethics of research using DNA recombination
Lesson 4
Gene cloning method using plasmid vector
Principle of preparation for genome library
Lesson 5
Principle of preparation for cDNA library
Methods for analyzing gene expression
Lesson 6
Principle and characteristics of E. coli expression system
Lesson 7
Summarize Lessons 1～6
Lesson 8
Principle and characteristics of various protein expression systems
Lesson 9
Characteristics of biopharmaceutical
Lesson 10
Principle of DNA sequencing
Lesson 11
Principle of PCR
Lesson 12
Application methods using PCR in pharmaceutical sciences
Lesson 13
Principle of methods for introduction of DNA into mammalian cells
Lesson 14
The way of practical gene therapy
Lesson 15
Summarize Lessons 8～14

Requirements None

Grading Method Mid-term examination (100 points), Term-end examination (100 points), total 200 points. A passing score is 120 points or up.

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message Questions are acceptable by E-mail.

Other In this lecture, you can achieve contents of the following SBOs.
C6-(4)-②-1, C6-(4)-②-2, C6-(4)-②-3, C6-(4)-④-1, C6-(4)-④-2, C6-(4)-④-3, C6-(4)-④-4, C6-(4)-④-5, C6-(4)-⑥-1, C6-(4)-⑥-2, C7-(1)-①-1, C7-(1)-①-2, C7-(1)-①-3, C8-(3)-②-1, C8-(3)-②-2, C8-(3)-②-3, C8-(3)-②-4, C8-(3)-②-5, C8-(3)-②-6, E2-(8)-②-1

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2025Year	School/Graduate School	School of Pharmaceutical Sciences Program of Pharmaceutical Sciences
Lecture Code	I7062002	Subject Classification	Specialized Education
Subject Name	遺伝子工学
Subject Name （Katakana）	イデンシコウガク
Subject Name in English	Genetic Engineering
Instructor	KUMAGAI TAKANORI
Instructor (Katakana)	クマガイ　タカノリ
Campus	Kasumi	Semester/Term	3rd-Year, Second Semester, Intensive
Days, Periods, and Classrooms	(Int) Inte：霞R404講義室
Lesson Style	Lecture	Lesson Style (More Details)	Face-to-face
Lecture
Credits	2.0	Class Hours/Week		Language of Instruction	B : Japanese／English
Course Level	3 : Undergraduate High-Intermediate
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	04 : Life Sciences
Eligible Students
Keywords	restriction enzyme, vector, host, cloning, library, hybridization, DNA sequence, polymerase chain reaction, mutagenesis, gene introduction, gene therapy, genome
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)	The second step: Structure and function of organisms
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Pharmaceutical Sciences （Knowledge and Understanding）・Knowledge and understanding of the biological maintenance system of homeostasis and the ability to adjust to the environment ●quality⑤ Medicinal Sciences （Knowledge and Understanding）・Knowledge of human and biological bodies. （Abilities and Skills）・Development of knowledge about human and biological bodies. (advance) ・To be able to basically treat major chemical agents, substances related to the living body, and microbes.
Class Objectives /Class Outline	Understand the basic of genetic manipulation and study applicatios of genetic engineering in pharmaceutical field
Class Schedule	lesson 1 ○Guidance ○History of genetic engineering lesson 2 ○Tools for genetic engineering (1) 　Restriction enzymes 　Modification enzymes 　Vectors lesson 3 ○Tools for genetic engineering (2) 　Hosts ○Cartagena protocol on Biosafety lesson4 ○Gene cloning (1) 　Cloning with plasmid vector 　Preparation of phage library 　Preparation of cosmid library lesson 5 ○Gene cloning (2) 　Preparation of cDNA library ○Analysis of gene expression (Northern analysis, Western analysis, etc.) lesson 6 ○Overproduction of proteins (1) 　E. coli expression system lesson 7 ○Summary (Lessons 1～6) lesson 8 ○Overproduction of proteins (2) 　Brevibacillus expression system 　Yeast expression system 　Baculovirus expression system 　Mammalian expression system 　Cell-free expression system lesson 9 ○Biopharmaceutical lesson 10 ○DNA sequencing 　Sanger sequencing 　Next generation sequencing lesson 11 ○Polymerase chain reaction (PCR) and its application (1) 　Principle of PCR lesson 12 ○PCR and its application (2) 　Application of PCR lesson 13 ○Mutagenesis to DNA 　Random mutagenesis 　Site-directed mutagenesis ○Introduction of DNA into mammalian cells and gene therapy (1) 　Introduction of DNA into mammalian cells lesson 14 ○Introduction of DNA into mammalian cells and gene therapy (2) 　Gene therapy lesson 15 ○Summary (Lessons 8～14)
Text/Reference Books,etc.	Bioscience of the gene and protein (Sankyo Shuppan)
PC or AV used in Class,etc.	Handouts, Other (see [More Details]), moodle
(More Details)	Original prints, PowerPoint. Video of lectures are uploaded on moodle (for review).
Learning techniques to be incorporated
Suggestions on Preparation and Review	Understand well the following points Lesson 1 Experiments leading to the development of genetic engineering Lesson 2 Characteristics of enzymes and vectors used for genetic engineering Lesson 3 Characteristics of hosts used for genetic engineering Ethics of research using DNA recombination Lesson 4 Gene cloning method using plasmid vector Principle of preparation for genome library Lesson 5 Principle of preparation for cDNA library Methods for analyzing gene expression Lesson 6 Principle and characteristics of E. coli expression system Lesson 7 Summarize Lessons 1～6 Lesson 8 Principle and characteristics of various protein expression systems Lesson 9 Characteristics of biopharmaceutical Lesson 10 Principle of DNA sequencing Lesson 11 Principle of PCR Lesson 12 Application methods using PCR in pharmaceutical sciences Lesson 13 Principle of methods for introduction of DNA into mammalian cells Lesson 14 The way of practical gene therapy Lesson 15 Summarize Lessons 8～14
Requirements	None
Grading Method	Mid-term examination (100 points), Term-end examination (100 points), total 200 points. A passing score is 120 points or up.
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message	Questions are acceptable by E-mail.
Other	In this lecture, you can achieve contents of the following SBOs. C6-(4)-②-1, C6-(4)-②-2, C6-(4)-②-3, C6-(4)-④-1, C6-(4)-④-2, C6-(4)-④-3, C6-(4)-④-4, C6-(4)-④-5, C6-(4)-⑥-1, C6-(4)-⑥-2, C7-(1)-①-1, C7-(1)-①-2, C7-(1)-①-3, C8-(3)-②-1, C8-(3)-②-2, C8-(3)-②-3, C8-(3)-②-4, C8-(3)-②-5, C8-(3)-②-6, E2-(8)-②-1
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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