TB053103 Start-Up Coursework of Biomedical Sciences

Hiroshima University Syllabus

Japanese

Academic Year 2024Year School/Graduate School Graduate School of Biomedical and Health Sciences (Master’s Course)

Lecture Code TB053103 Subject Classification Specialized Education

Subject Name スタートアップ生命科学コースワーク

Subject Name
（Katakana）スタートアップセイメイカガクコースワーク

Subject Name in
English Start-Up Coursework of Biomedical Sciences

Instructor CHAYAMA HIROMI

Instructor
(Katakana) チャヤマ　ヒロミ

Campus Kasumi Semester/Term 1st-Year, Second Semester, 3Term

Days, Periods, and Classrooms (3T) Inte

Lesson Style Lecture Lesson Style
(More Details) 　

lecture style etc...

Credits 1.0 Class Hours/Week 　 Language of Instruction B : Japanese／English

Course Level 5 : Graduate Basic

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 04 : Life Sciences

Eligible Students

Keywords

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students)

Class Objectives
/Class Outline Students will learn basic experimental knowledge and techniques for medical and biological research in small groups.

Class Schedule There are eight practices.
1 Wednesday, October 2nd, 9:30-12:30
"Review of molecular biology"
Students will understand DNA,RNA and protein structures, molecular genetics and the biochemistry of transcription and protein synthesis. Each student receives the assignment from Teams and completes it at home or in the lab. You are required to provide your laptop and writing implements.

2 Wednesday, October 9th, 9:30-12:30
"Transfection DNA Plasmid into Mammalian Cells”
Students can carry out basic cell-culture and transfection techniques properly and safely on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.

3 Tuesday, October 15th, 9:30-12:30
"Protein gel electrophoresis part1”
Students will learn about principle and technique of protein gel electrophoresis using SDS-PAGE. In addition, they will set up gels and perform on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.

4 Wednesday, October 16th, 9:30-12:30
"Protein gel electrophoresis part2”
Students will detect targeted protein expression using ECL reagents with the ChemiDoc imaging system on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.

5 Wednesday, October 23rd, 9:30-12:30
"Purification of nucleic acid”
Students will learn about principle and technique of nucleic acid purification, reverse transcription and cDNA synthesis on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.

6 Wednesday, October 30th, 9:30-12:30
"PCR, agarose gel electrophoresis”
Students will design primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.

7 Wednesday, November 6th, 9:30-12:30
"Real time PCR”
Students will quantify the expression levels of the target gene and endogenous control gene by real time PCR. on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements.

8 Wednesday, November 13th, 9:30-12:30
"Data analysis and review of the class”
Students will learn about principle and technique of mRNA expression level quantification by real time PCR. Each student receives the assignment from Teams and completes it at home or in the lab. You are required to provide your laptop and writing implements.

Text/Reference
Books,etc. Handouts

PC or AV used in
Class,etc. 　

(More Details) Laptop

Learning techniques to be incorporated 　

Suggestions on
Preparation and
Review The day before each class, handouts will be uploaded to Teams. Please make use of them for class preparation and review.

Requirements Please make sure the class date, time, venue and what you need for the class.
If you have further questions, please contact Professor Hiromi Chayama: habe@hiroshima-u.ac.jp.

Grading Method Students will be evaluated by their assignments.

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message

Other Exercises are usually done face-to-face, but depending on the number of participants and the social context, they can also be done in a parallel, interactive online format or via video streaming.
You will be invited to join Teams after registering for the course, so please check your "hirodai-mail"(account name or arbitrary character string @hiroshima-u.ac.jp).

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2024Year	School/Graduate School	Graduate School of Biomedical and Health Sciences (Master’s Course)
Lecture Code	TB053103	Subject Classification	Specialized Education
Subject Name	スタートアップ生命科学コースワーク
Subject Name （Katakana）	スタートアップセイメイカガクコースワーク
Subject Name in English	Start-Up Coursework of Biomedical Sciences
Instructor	CHAYAMA HIROMI
Instructor (Katakana)	チャヤマ　ヒロミ
Campus	Kasumi	Semester/Term	1st-Year, Second Semester, 3Term
Days, Periods, and Classrooms	(3T) Inte
Lesson Style	Lecture	Lesson Style (More Details)
lecture style etc...
Credits	1.0	Class Hours/Week		Language of Instruction	B : Japanese／English
Course Level	5 : Graduate Basic
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	04 : Life Sciences
Eligible Students
Keywords
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)
Class Objectives /Class Outline	Students will learn basic experimental knowledge and techniques for medical and biological research in small groups.
Class Schedule	There are eight practices. 1 Wednesday, October 2nd, 9:30-12:30 "Review of molecular biology" Students will understand DNA,RNA and protein structures, molecular genetics and the biochemistry of transcription and protein synthesis. Each student receives the assignment from Teams and completes it at home or in the lab. You are required to provide your laptop and writing implements. 2 Wednesday, October 9th, 9:30-12:30 "Transfection DNA Plasmid into Mammalian Cells” Students can carry out basic cell-culture and transfection techniques properly and safely on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements. 3 Tuesday, October 15th, 9:30-12:30 "Protein gel electrophoresis part1” Students will learn about principle and technique of protein gel electrophoresis using SDS-PAGE. In addition, they will set up gels and perform on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements. 4 Wednesday, October 16th, 9:30-12:30 "Protein gel electrophoresis part2” Students will detect targeted protein expression using ECL reagents with the ChemiDoc imaging system on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements. 5 Wednesday, October 23rd, 9:30-12:30 "Purification of nucleic acid” Students will learn about principle and technique of nucleic acid purification, reverse transcription and cDNA synthesis on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements. 6 Wednesday, October 30th, 9:30-12:30 "PCR, agarose gel electrophoresis” Students will design primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements. 7 Wednesday, November 6th, 9:30-12:30 "Real time PCR” Students will quantify the expression levels of the target gene and endogenous control gene by real time PCR. on the 6th floor of Building A. Please come to the front of room A610 at 9:30 a.m. You are required to provide your lab coat and writing implements. 8 Wednesday, November 13th, 9:30-12:30 "Data analysis and review of the class” Students will learn about principle and technique of mRNA expression level quantification by real time PCR. Each student receives the assignment from Teams and completes it at home or in the lab. You are required to provide your laptop and writing implements.
Text/Reference Books,etc.	Handouts
PC or AV used in Class,etc.
(More Details)	Laptop
Learning techniques to be incorporated
Suggestions on Preparation and Review	The day before each class, handouts will be uploaded to Teams. Please make use of them for class preparation and review.
Requirements	Please make sure the class date, time, venue and what you need for the class. If you have further questions, please contact Professor Hiromi Chayama: habe@hiroshima-u.ac.jp.
Grading Method	Students will be evaluated by their assignments.
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message
Other	Exercises are usually done face-to-face, but depending on the number of participants and the social context, they can also be done in a parallel, interactive online format or via video streaming. You will be invited to join Teams after registering for the course, so please check your "hirodai-mail"(account name or arbitrary character string @hiroshima-u.ac.jp).
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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