Hiroshima University Syllabus

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Academic Year 2024Year School/Graduate School School of Pharmaceutical Sciences Program of Pharmaceutical Sciences
Lecture Code I7096001 Subject Classification Specialized Education
Subject Name 細胞分子生物学実習
Subject Name
Subject Name in
Experiments of Cellular and Molecular Biology
タハラ ヒデトシ,タカハシ リョウウ,ヤマモト ユウキ
Campus Kasumi Semester/Term 2nd-Year,  Second Semester,  Second Semester
Days, Periods, and Classrooms (2nd) Inte
Lesson Style Practical Lesson Style
(More Details)
Experiments using chemical reagents 
Credits 1.0 Class Hours/Week   Language of Instruction B : Japanese/English
Course Level 2 : Undergraduate Low-Intermediate
Course Area(Area) 27 : Health Sciences
Course Area(Discipline) 03 : Pharmaceutical Sciences
Eligible Students Second- year students
Keywords cell culture, aseptic manipulation, microscopy, genomic DNA, radioisotope, Southern analysis 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
(Applicable only to targeted subjects for undergraduate students)
Pharmaceutical Sciences
(Comprehensive Abilities)
・The research ability: the ability to select issues to be solved in the professional field of pharmacist and carry out measures and research to solve the issues.  ●quality ⑧

Medicinal Sciences
(Abilities and Skills)
・To be able to basically treat major chemical agents, substances related to the living body, and microbes. 
Class Objectives
/Class Outline
Basics of cell culture
Isolation and quantification of human genomic DNA
Gene analysis using electrophoresis and hybridization of radioisotope labeled probe 
Class Schedule Experiment A
Isolation and quantification of genomic DNA from human peripheral blood
Experiment B
Southern analysis: Detection of telomeric DNA sequence of human chromosome ends using radioisotope labeled probe
Experiment C
Basics of cell culture (aseptic manipulation, subculture, cell count, cultured cells morphology, chromosomes morphology depending on the cell cycle, observations using the microscopy)

After the course work, present laboratory notebooks describing experimental protocols, results, observations and answers to questions in the textbook. 
The special textbook will be distributed. 
PC or AV used in
(More Details) Video (audio and subtitle) 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Prepare protocols on your laboratory notebook before experiments.
Review your knowledge about cells, DNA, genes, radioisotope and basic experimental technique. 
Requirements Experiments using radioisotope must be done in compliance with The Act Concerning Prevention from Radiation Hazards due to Radioisotopes, etc. which is requiring a prior medical check-up. 
Grading Method Evaluation points are the proficiency level estimate from laboratory notebooks, participation and attendance in class. 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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