| Academic Year |
2024Year |
School/Graduate School |
School of Pharmaceutical Sciences Program of Pharmaceutical Sciences |
| Lecture Code |
I7096001 |
Subject Classification |
Specialized Education |
| Subject Name |
細胞分子生物学実習 |
Subject Name
（Katakana） |
サイボウブンシセイブツガクジッシュウ |
Subject Name in
English |
Experiments of Cellular and Molecular Biology |
| Instructor |
TAHARA HIDETOSHI,TAKAHASHI RYOUU,YAMAMOTO YUKI |
Instructor
(Katakana) |
タハラ ヒデトシ,タカハシ リョウウ,ヤマモト ユウキ |
| Campus |
Kasumi |
Semester/Term |
2nd-Year, Second Semester, Second Semester |
| Days, Periods, and Classrooms |
(2nd) Inte |
| Lesson Style |
Practical |
Lesson Style
(More Details) |
|
| Experiments using chemical reagents |
| Credits |
1.0 |
Class Hours/Week |
|
Language of Instruction |
B
:
Japanese／English |
| Course Level |
2
:
Undergraduate Low-Intermediate
|
| Course Area（Area） |
27
:
Health Sciences |
| Course Area（Discipline） |
03
:
Pharmaceutical Sciences |
| Eligible Students |
Second- year students |
| Keywords |
cell culture, aseptic manipulation, microscopy, genomic DNA, radioisotope, Southern analysis |
| Special Subject for Teacher Education |
|
Special Subject |
|
Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) | |
|---|
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) | Pharmaceutical Sciences
（Comprehensive Abilities）
・The research ability: the ability to select issues to be solved in the professional field of pharmacist and carry out measures and research to solve the issues. ●quality ⑧
Medicinal Sciences
（Abilities and Skills）
・To be able to basically treat major chemical agents, substances related to the living body, and microbes. |
|---|
Class Objectives
/Class Outline |
Basics of cell culture
Isolation and quantification of human genomic DNA
Gene analysis using electrophoresis and hybridization of radioisotope labeled probe |
| Class Schedule |
Experiment A
Isolation and quantification of genomic DNA from human peripheral blood
Experiment B
Southern analysis: Detection of telomeric DNA sequence of human chromosome ends using radioisotope labeled probe
Experiment C
Basics of cell culture (aseptic manipulation, subculture, cell count, cultured cells morphology, chromosomes morphology depending on the cell cycle, observations using the microscopy)
After the course work, present laboratory notebooks describing experimental protocols, results, observations and answers to questions in the textbook. |
Text/Reference
Books,etc. |
The special textbook will be distributed. |
PC or AV used in
Class,etc. |
|
| (More Details) |
Video (audio and subtitle) |
| Learning techniques to be incorporated |
|
Suggestions on
Preparation and
Review |
Prepare protocols on your laboratory notebook before experiments.
Review your knowledge about cells, DNA, genes, radioisotope and basic experimental technique. |
| Requirements |
Experiments using radioisotope must be done in compliance with The Act Concerning Prevention from Radiation Hazards due to Radioisotopes, etc. which is requiring a prior medical check-up. |
| Grading Method |
Evaluation points are the proficiency level estimate from laboratory notebooks, participation and attendance in class. |
| Practical Experience |
|
| Summary of Practical Experience and Class Contents based on it |
|
| Message |
|
| Other |
|
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. |