Japanese

Academic Year	2024Year	School/Graduate School	School of Pharmaceutical Sciences Program of Pharmaceutical Sciences
Lecture Code	I7062002	Subject Classification	Specialized Education
Subject Name	遺伝子工学
Subject Name （Katakana）	イデンシコウガク
Subject Name in English	Genetic Engineering
Instructor	KUMAGAI TAKANORI
Instructor (Katakana)	クマガイ　タカノリ
Campus	Kasumi	Semester/Term	3rd-Year,  Second Semester,  Intensive
Days, Periods, and Classrooms	(Int) Inte：PHS Lecture Rm 3
Lesson Style	Lecture	Lesson Style (More Details)
			Lecture
Credits	2.0	Class Hours/Week		Language of Instruction	B : Japanese／English
Course Level	3 : Undergraduate High-Intermediate
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	04 : Life Sciences
Eligible Students
Keywords	restriction enzyme, vector, host, cloning, library, hybridization, DNA sequence, polymerase chain reaction, mutagenesis, gene introduction, gene therapy, genome
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)	The second step: Structure and function of organisms
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Pharmaceutical Sciences （Knowledge and Understanding） ・Knowledge and understanding of the biological maintenance system of homeostasis and the ability to adjust to the environment  ●quality⑤ Medicinal Sciences （Knowledge and Understanding） ・Knowledge of human and biological bodies. （Abilities and Skills） ・Development of knowledge about human and biological bodies. (advance) ・To be able to basically treat major chemical agents, substances related to the living body, and microbes.
Class Objectives /Class Outline	Understand the basic of genetic manipulation and study applicatios of genetic engineering in pharmaceutical field
Class Schedule	lesson 1 ○ Guidance ○ History of genetic engineering lesson 2 ○ Tools for genetic engineering (1)    Restriction enzymes, modification enzymes lesson 3 ○ Cartagena protocol on Biosafety lesson4 ○ Tools for genetic engineering (2)    Vectors, hosts lesson 5 ○ Cloning (1)    Southern analysis, genomic library, cDNA library lesson 6 ○ Cloning (2)    Colony hybridization, plaque hybridization ○ Analysis of gene expression (Northern analysis, Western analysis) lesson 7 ○ Summary lesson 8 ○ Overproduction of proteins lesson 9 ○ DNA sequencing lesson 10 ○ Polymerase chain reaction and its applications lesson 11 ○ Mutagenesis to DNA    Random mutation, site-directed mutation lesson 12 ○ Introduction of DNA into mammalian cells lesson 13 ○ Gene therapy lesson 14 ○ Application of bacterial genome information lesson 15 ○ Summary
Text/Reference Books,etc.	Bioscience of the gene and protein (Sankyo Shuppan)
PC or AV used in Class,etc.
	(More Details)	Original prints, PowerPoint. Video of lectures are uploaded on moodle (for review).
Learning techniques to be incorporated
Suggestions on Preparation and Review	Understand well the following points: Lesson 1 Experiments leading to the development of genetic engineering Lesson 2 Characteristics of enzymes used for genetic engineering Lesson 3 Ethic of research using DNA recombination Lesson 4 Characteristics of vectors and hosts used for genetic engineering Lesson 5 Principle of preparation for genomic and cDNA libraries Lesson 6 Principle of various hybridization methods and their applications Lesson 7 Well summarize lessons 1 - 6 Lesson 8 Principle and characteristics of protein expression in bacteria Lesson 9 Principle of DNA sequencing Lesson 10 Principle of polymerase chain reaction and its applications in Pharmaceutical Sciences Lesson 11 Principle of mutagenesis to DNA Lesson 12 Principle of methods for introduction of DNA into mammalian cells Lesson 13 The way of practical gene therapy Lesson 14 Applications Lesson 15 Well summarize lessons 8 - 14
Requirements	None
Grading Method	Mid-term examination (100 points), Term-end examination (100 points), total 200 points. A passing score is 120 points or up.
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message	Questions are acceptable by E-mail.
Other	In this lecture, you can achieve contents of the following SBOs. C6-(4)-②-1, C6-(4)-②-2, C6-(4)-②-3, C6-(4)-④-1, C6-(4)-④-2, C6-(4)-④-3, C6-(4)-④-4, C6-(4)-④-5, C6-(4)-⑥-1, C6-(4)-⑥-2, C7-(1)-①-1, C7-(1)-①-2, C7-(1)-①-3, C8-(3)-②-1, C8-(3)-②-2, C8-(3)-②-3, C8-(3)-②-4, C8-(3)-②-5, C8-(3)-②-6, E2-(8)-②-1
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