Hiroshima University Syllabus

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Academic Year 2024Year School/Graduate School School of Pharmaceutical Sciences Program of Pharmaceutical Sciences
Lecture Code I7062001 Subject Classification Specialized Education
Subject Name 遺伝子工学
Subject Name
Subject Name in
Genetic Engineering
クマガイ タカノリ
Campus Kasumi Semester/Term 3rd-Year,  Second Semester,  3Term
Days, Periods, and Classrooms (3T) Weds1-4:霞R404講義室
Lesson Style Lecture Lesson Style
(More Details)
Credits 2.0 Class Hours/Week   Language of Instruction B : Japanese/English
Course Level 3 : Undergraduate High-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 04 : Life Sciences
Eligible Students
Keywords restriction enzyme, vector, host, cloning, library, hybridization, DNA sequence, polymerase chain reaction, mutagenesis, gene introduction, gene therapy, genome 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
(Applicable only to targeted subjects for undergraduate students)
The second step: Structure and function of organisms 
Criterion referenced
(Applicable only to targeted subjects for undergraduate students)
Pharmaceutical Sciences
(Knowledge and Understanding)
・Knowledge and understanding of the biological maintenance system of homeostasis and the ability to adjust to the environment  ●quality⑤

Medicinal Sciences
(Knowledge and Understanding)
・Knowledge of human and biological bodies.
(Abilities and Skills)
・Development of knowledge about human and biological bodies. (advance)
・To be able to basically treat major chemical agents, substances related to the living body, and microbes. 
Class Objectives
/Class Outline
Understand the basic of genetic manipulation and study applicatios of genetic engineering in pharmaceutical field 
Class Schedule lesson 1
○ Guidance
○ History of genetic engineering
lesson 2
○ Tools for genetic engineering (1)
   Restriction enzymes, modification enzymes
lesson 3
○ Cartagena protocol on Biosafety
○ Tools for genetic engineering (2)
   Vectors, hosts
lesson 5
○ Cloning (1)
   Southern analysis, genomic library, cDNA library
lesson 6
○ Cloning (2)
   Colony hybridization, plaque hybridization
○ Analysis of gene expression (Northern analysis, Western analysis)
lesson 7
○ Summary
lesson 8
○ Overproduction of proteins
lesson 9
○ DNA sequencing
lesson 10
○ Polymerase chain reaction and its applications
lesson 11
○ Mutagenesis to DNA
   Random mutation, site-directed mutation
lesson 12
○ Introduction of DNA into mammalian cells
lesson 13
○ Gene therapy
lesson 14
○ Application of bacterial genome information
lesson 15
○ Summary 
Bioscience of the gene and protein (Sankyo Shuppan) 
PC or AV used in
(More Details) Original prints, PowerPoint.
Video of lectures are uploaded on moodle (for review). 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Understand well the following points:
Lesson 1
Experiments leading to the development of genetic engineering
Lesson 2
Characteristics of enzymes used for genetic engineering
Lesson 3
Ethic of research using DNA recombination
Lesson 4
Characteristics of vectors and hosts used for genetic engineering
Lesson 5
Principle of preparation for genomic and cDNA libraries
Lesson 6
Principle of various hybridization methods and their applications
Lesson 7
Well summarize lessons 1 - 6
Lesson 8
Principle and characteristics of protein expression in bacteria
Lesson 9
Principle of DNA sequencing
Lesson 10
Principle of polymerase chain reaction and its applications in Pharmaceutical Sciences
Lesson 11
Principle of mutagenesis to DNA
Lesson 12
Principle of methods for introduction of DNA into mammalian cells
Lesson 13
The way of practical gene therapy
Lesson 14
Lesson 15
Well summarize lessons 8 - 14 
Requirements None 
Grading Method Mid-term examination (100 points), Term-end examination (100 points), total 200 points. A passing score is 120 points or up. 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message Questions are acceptable by E-mail. 
Other In this lecture, you can achieve contents of the following SBOs.
C6-(4)-②-1, C6-(4)-②-2, C6-(4)-②-3, C6-(4)-④-1, C6-(4)-④-2, C6-(4)-④-3, C6-(4)-④-4, C6-(4)-④-5, C6-(4)-⑥-1, C6-(4)-⑥-2, C7-(1)-①-1, C7-(1)-①-2, C7-(1)-①-3, C8-(3)-②-1, C8-(3)-②-2, C8-(3)-②-3, C8-(3)-②-4, C8-(3)-②-5, C8-(3)-②-6, E2-(8)-②-1 
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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