HL020000 Practice for Fundamental Biology II

Hiroshima University Syllabus

Japanese

Academic Year 2024Year School/Graduate School School of Science

Lecture Code HL020000 Subject Classification Specialized Education

Subject Name 生物科学基礎実験II

Subject Name
（Katakana）セイブツカガクキソジッケン２

Subject Name in
English Practice for Fundamental Biology II

Instructor SAKAMOTO NAOAKI,IGAWA TAKESHI,IMAMURA TAKUYA,SUZUKI MAKOTO,KUSABA MAKOTO,OKUMURA MISAKO,TOYOKURA KOICHI,HOSOBA KOSUKE,MORISHITA FUMIHIRO,HANADA HIDEKI,NAKATSUBO KEIKO,TSUBOTA HIROMI,HAMAO KOZUE,HONDA MIZUKI,UEKI TATSUYA

Instructor
(Katakana) サカモト　ナオアキ,イガワ　タケシ,イマムラ　タクヤ,スズキ　マコト,クサバ　マコト,オクムラ　ミサコ,トヨクラ　コウイチ,ホソバ　コウスケ,モリシタ　フミヒロ,ハナダ　ヒデキ,ナカツボ　ケイコ,ツボタ　ヒロミ,ハマオ　コズエ,ホンダ　ミズキ,ウエキ　タツヤ

Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  Second Semester

Days, Periods, and Classrooms (2nd) Tues7-10,Thur7-10：East Library 2F BYOD Rm

Lesson Style Experiment Lesson Style
(More Details) 　

Experiment, field work, use of chemicals

Credits 4.0 Class Hours/Week 　 Language of Instruction J : Japanese

Course Level 3 : Undergraduate High-Intermediate

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 03 : Basic Biology

Eligible Students 2nd-year students of the Department of Biological Science

Keywords structure and function of cell, gene mapping and chromosome, animal response, bioinformatics, gene and transformation, sea urchin development

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students) Students learn skills for biological observation and experimental manipulation and abilities to evaluate experimental results and data with scientific view points.
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) Biology
（Abilities and Skills）
・Based on basic knowledge which is already acquired, to obtain the following experimental skills in order to practice research: 1) Basic observation skills and skills to manage experiments. 2) Ability to record observed natural phenomena. 3) Ability to collect and assess data.

Class Objectives
/Class Outline Students will learn experimental procedures for basic molecular biology, basic developmental biology, cell biology, genetics, animal physiology and bioinformatics as the basis of the study of biological sciences.

Class Schedule 1. General guidance (all instructors, SHIMAMURA MASAKI)
2. Cell biology (1) Design of experimental plan (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE)
3. Cell biology (2) Indirect immunofluorescent staining (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE)
4. Cell biology (3) Observation by fluorescent microscopy (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE)
5. Cell biology (4) SDS-polyacrylamide gel electrophoresis (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE)
6. Cell biology (5) Immunoblotting (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE)
7. Cell biology (6) Discussion (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE)
8. Bioinformatics (1) Retrieval of references (MORISHITA FUMIHIRO, IGAWA TAKESHI)
9. Bioinformatics (2) Homology search on protein and nucleotide databases (MORISHITA FUMIHIRO, IGAWA TAKESHI)
10. Bioinformatics (3) Alignment and molecular phylogenetic analysis (MORISHITA FUMIHIRO, IGAWA TAKESHI)
11. Bioinformatics (4) Basis of biological statistics (TSUBOTA HIROMI, BONO HIDEMASA)
12. Bioinformatics (5) Basis of R and basic statistics (TSUBOTA HIROMI, BONO HIDEMASA)
13. Bioinformatics (6) Multivariate analysis by R (TSUBOTA HIROMI, BONO HIDEMASA)
14. Animal physiology (1) Neural regulation (UEKI TATSUYA, MORISHITA FUMIHIRO, HANADA HIDEKI)
15. Animal physiology (2) Hormonal regulation (UEKI TATSUYA, MORISHITA FUMIHIRO, HANADA HIDEKI)
16. Animal physiology (3) Responses of the invertebrates (UEKI TATSUYA, MORISHITA FUMIHIRO, HANADA HIDEKI)
17. Animal physiology (4) Discussion and presentation (UEKI TATSUYA, MORISHITA FUMIHIRO, HANADA HIDEKI)
18. Genetics (1) RNA-seq analysis of human neural stem cell using public data  (IMAMURA TAKUYA, IGAWA TAKESHI, SUZUKI MAKOTO, HONDA MIZUKI)
19. Genetics (2) RNA-seq analysis of human neural stem cell using public data (IMAMURA TAKUYA, IGAWA TAKESHI, SUZUKI MAKOTO, HONDA MIZUKI)
20. Genetics (3) Discussion (IMAMURA TAKUYA, IGAWA TAKESHI, SUZUKI MAKOTO, HONDA MIZUKI)
21. Basic molecular biology (1) Preparation of reagents (SAKAMOTO NAOAKI, NAKATSUBO KEIKO)
22. Basic molecular biology (2) Transformation (SAKAMOTO NAOAKI, NAKATSUBO KEIKO)
23. Basic molecular biology (3) Restriction enzyme digestion and agarose gel electrophoresis (SAKAMOTO NAOAKI, NAKATSUBO KEIKO)
24. Basic molecular biology (4) Extraction of plasmid DNA (SAKAMOTO NAOAKI, NAKATSUBO KEIKO)
25. Genetics (4) Observation and selection of Arabidopsis mutants (KUSABA MAKOTO, TOYOKURA KOICHI)
26. Genetics (5) Gene mapping (1) (KUSABA MAKOTO, TOYOKURA KOICHI)
27. Genetics (6) Gene mapping (2) (KUSABA MAKOTO, TOYOKURA KOICHI)
28. Basic developmental biology (1) Observation of sea urchin development (I) (SAKAMOTO NAOAKI, NAKATSUBO KEIKO)
29. Basic developmental biology (2) Observation of sea urchin development (II) (SAKAMOTO NAOAKI, NAKATSUBO KEIKO)
30. Basic developmental biology (3) Extraction of RNA from sea urchin embryos (SAKAMOTO NAOAKI, NAKATSUBO KEIKO)

Text/Reference
Books,etc. Textbook for "Practice for Fundamental Biology"
Some reference materials may be distributed for each experiment.

PC or AV used in
Class,etc. 　

(More Details) Textbook, PC, research equipments

Learning techniques to be incorporated 　

Suggestions on
Preparation and
Review Textbook for "Practice for Fundamental Biology" should be read before and after each experiment.
Report of each experiment should be prepared immediately after experiment.
Each report should include (1) purpose, (2) experimental procedures, (3) results and (4) discussions.

Requirements Students have to attend all experiments and any truancy is not allowed.

Grading Method The grade will be evaluated by reports (80%) and attitude (20%).

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message Because each experiment may involve the use of hazardous materials, students need to pay enough attention to the experimental procedures.

Other

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2024Year	School/Graduate School	School of Science
Lecture Code	HL020000	Subject Classification	Specialized Education
Subject Name	生物科学基礎実験II
Subject Name （Katakana）	セイブツカガクキソジッケン２
Subject Name in English	Practice for Fundamental Biology II
Instructor	SAKAMOTO NAOAKI,IGAWA TAKESHI,IMAMURA TAKUYA,SUZUKI MAKOTO,KUSABA MAKOTO,OKUMURA MISAKO,TOYOKURA KOICHI,HOSOBA KOSUKE,MORISHITA FUMIHIRO,HANADA HIDEKI,NAKATSUBO KEIKO,TSUBOTA HIROMI,HAMAO KOZUE,HONDA MIZUKI,UEKI TATSUYA
Instructor (Katakana)	サカモト　ナオアキ,イガワ　タケシ,イマムラ　タクヤ,スズキ　マコト,クサバ　マコト,オクムラ　ミサコ,トヨクラ　コウイチ,ホソバ　コウスケ,モリシタ　フミヒロ,ハナダ　ヒデキ,ナカツボ　ケイコ,ツボタ　ヒロミ,ハマオ　コズエ,ホンダ　ミズキ,ウエキ　タツヤ
Campus	Higashi-Hiroshima	Semester/Term	2nd-Year, Second Semester, Second Semester
Days, Periods, and Classrooms	(2nd) Tues7-10,Thur7-10：East Library 2F BYOD Rm
Lesson Style	Experiment	Lesson Style (More Details)
Experiment, field work, use of chemicals
Credits	4.0	Class Hours/Week		Language of Instruction	J : Japanese
Course Level	3 : Undergraduate High-Intermediate
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	03 : Basic Biology
Eligible Students	2nd-year students of the Department of Biological Science
Keywords	structure and function of cell, gene mapping and chromosome, animal response, bioinformatics, gene and transformation, sea urchin development
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)	Students learn skills for biological observation and experimental manipulation and abilities to evaluate experimental results and data with scientific view points.
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Biology （Abilities and Skills）・Based on basic knowledge which is already acquired, to obtain the following experimental skills in order to practice research: 1) Basic observation skills and skills to manage experiments. 2) Ability to record observed natural phenomena. 3) Ability to collect and assess data.
Class Objectives /Class Outline	Students will learn experimental procedures for basic molecular biology, basic developmental biology, cell biology, genetics, animal physiology and bioinformatics as the basis of the study of biological sciences.
Class Schedule	1. General guidance (all instructors, SHIMAMURA MASAKI) 2. Cell biology (1) Design of experimental plan (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE) 3. Cell biology (2) Indirect immunofluorescent staining (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE) 4. Cell biology (3) Observation by fluorescent microscopy (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE) 5. Cell biology (4) SDS-polyacrylamide gel electrophoresis (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE) 6. Cell biology (5) Immunoblotting (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE) 7. Cell biology (6) Discussion (HAMAO KOZUE, OKUMURA MISAKO, HOSOBA KOSUKE) 8. Bioinformatics (1) Retrieval of references (MORISHITA FUMIHIRO, IGAWA TAKESHI) 9. Bioinformatics (2) Homology search on protein and nucleotide databases (MORISHITA FUMIHIRO, IGAWA TAKESHI) 10. Bioinformatics (3) Alignment and molecular phylogenetic analysis (MORISHITA FUMIHIRO, IGAWA TAKESHI) 11. Bioinformatics (4) Basis of biological statistics (TSUBOTA HIROMI, BONO HIDEMASA) 12. Bioinformatics (5) Basis of R and basic statistics (TSUBOTA HIROMI, BONO HIDEMASA) 13. Bioinformatics (6) Multivariate analysis by R (TSUBOTA HIROMI, BONO HIDEMASA) 14. Animal physiology (1) Neural regulation (UEKI TATSUYA, MORISHITA FUMIHIRO, HANADA HIDEKI) 15. Animal physiology (2) Hormonal regulation (UEKI TATSUYA, MORISHITA FUMIHIRO, HANADA HIDEKI) 16. Animal physiology (3) Responses of the invertebrates (UEKI TATSUYA, MORISHITA FUMIHIRO, HANADA HIDEKI) 17. Animal physiology (4) Discussion and presentation (UEKI TATSUYA, MORISHITA FUMIHIRO, HANADA HIDEKI) 18. Genetics (1) RNA-seq analysis of human neural stem cell using public data (IMAMURA TAKUYA, IGAWA TAKESHI, SUZUKI MAKOTO, HONDA MIZUKI) 19. Genetics (2) RNA-seq analysis of human neural stem cell using public data (IMAMURA TAKUYA, IGAWA TAKESHI, SUZUKI MAKOTO, HONDA MIZUKI) 20. Genetics (3) Discussion (IMAMURA TAKUYA, IGAWA TAKESHI, SUZUKI MAKOTO, HONDA MIZUKI) 21. Basic molecular biology (1) Preparation of reagents (SAKAMOTO NAOAKI, NAKATSUBO KEIKO) 22. Basic molecular biology (2) Transformation (SAKAMOTO NAOAKI, NAKATSUBO KEIKO) 23. Basic molecular biology (3) Restriction enzyme digestion and agarose gel electrophoresis (SAKAMOTO NAOAKI, NAKATSUBO KEIKO) 24. Basic molecular biology (4) Extraction of plasmid DNA (SAKAMOTO NAOAKI, NAKATSUBO KEIKO) 25. Genetics (4) Observation and selection of Arabidopsis mutants (KUSABA MAKOTO, TOYOKURA KOICHI) 26. Genetics (5) Gene mapping (1) (KUSABA MAKOTO, TOYOKURA KOICHI) 27. Genetics (6) Gene mapping (2) (KUSABA MAKOTO, TOYOKURA KOICHI) 28. Basic developmental biology (1) Observation of sea urchin development (I) (SAKAMOTO NAOAKI, NAKATSUBO KEIKO) 29. Basic developmental biology (2) Observation of sea urchin development (II) (SAKAMOTO NAOAKI, NAKATSUBO KEIKO) 30. Basic developmental biology (3) Extraction of RNA from sea urchin embryos (SAKAMOTO NAOAKI, NAKATSUBO KEIKO)
Text/Reference Books,etc.	Textbook for "Practice for Fundamental Biology" Some reference materials may be distributed for each experiment.
PC or AV used in Class,etc.
(More Details)	Textbook, PC, research equipments
Learning techniques to be incorporated
Suggestions on Preparation and Review	Textbook for "Practice for Fundamental Biology" should be read before and after each experiment. Report of each experiment should be prepared immediately after experiment. Each report should include (1) purpose, (2) experimental procedures, (3) results and (4) discussions.
Requirements	Students have to attend all experiments and any truancy is not allowed.
Grading Method	The grade will be evaluated by reports (80%) and attitude (20%).
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message	Because each experiment may involve the use of hazardous materials, students need to pay enough attention to the experimental procedures.
Other
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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