ANL19001 Practical Methods in Life Sciences C

Hiroshima University Syllabus

Japanese

Academic Year 2024Year School/Graduate School School of Integrated Arts and Sciences Department of Integrated Arts and Sciences

Lecture Code ANL19001 Subject Classification Specialized Education

Subject Name 生命科学実験法Ｃ

Subject Name
（Katakana）セイメイカガクジッケンホウC

Subject Name in
English Practical Methods in Life Sciences C

Instructor ISHIDA ATSUHIKO,ISHIHARA YASUHIRO,HIRANO TETSUO

Instructor
(Katakana) イシダ　アツヒコ,イシハラ　ヤスヒロ,ヒラノ　テツオ

Campus Higashi-Hiroshima Semester/Term 3rd-Year,  First Semester,  1Term

Days, Periods, and Classrooms (1T) Thur5,Fri5：IAS B414

Lesson Style Lecture Lesson Style
(More Details) 　

In this class students will develop their skills in biochemical and molecular biological experiments.

Credits 1.0 Class Hours/Week 　 Language of Instruction J : Japanese

Course Level 3 : Undergraduate High-Intermediate

Course Area（Area） 26 : Biological and Life Sciences

Course Area（Discipline） 04 : Life Sciences

Eligible Students

Keywords Protein, purification, enzyme activity, nucleic acid, plasmid DNA, transformation, restriction enzyme

Special Subject for Teacher Education 　 Special Subject 　

Class Status
within Educational
Program
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
Evaluation
(Applicable only to targeted subjects for undergraduate students) Integrated Arts and Sciences
（Abilities and Skills）
・The abilities and skills to summarize one's own research in reports or academic papers, and to deliver presentations at a seminar or research meetings, and to answer questions.

Class Objectives
/Class Outline This course enables students to master their skills in biochemical experiments through experiments in protein purification, fractionation of organelles, and enzymatic analysis. Basic experiments in molecular biology, such as transformation and PCR, are also carried out.
Students will develop their abilities to handle various biochemical samples, such as proteins and nucleic acids.

Class Schedule Lesson 1: Introduction to biochemical experiments
           (Ishida, Ishihara)
Lesson 2: Protein purification by ion-exchange chromatography
           (Ishida, Ishihara)
Lesson 3: Electrophoresis of proteins  (Ishida, Ishihara)
Lesson 4: Protein assay (Ishida, Ishihara)
Lesson 5: Fractionation of various organelles from rat liver
           (Ishida, Ishihara)
Lesson 6: Protein assay of the fractionated organelles
           (Ishida, Ishihara)
Lesson 7: Enzyme activity assay (1) (Ishida, Ishihara)
Lesson 8: Enzyme activity assay (2) (Ishida, Ishihara)
Lesson 9: Introduction to molecular biology and preparation of reagents and buffers  (Hirano, Ishida, Ishihara)
Lesson 10: Transformation of E. coli (Hirano, Ishida, Ishihara)
Lesson 11: Estimation of transformation efficiency, PCR, preparation of agarose gels  (Hirano, Ishida, Ishihara)
Lesson 12: Agarose gel electrophoresis of PCR products
           (Hirano, Ishida, Ishihara)
Lesson 13: Preparation of plasmid DNAs
           (Hirano, Ishida, Ishihara)
Lesson 14:Digestion of the plasmids by restriction enzymes
           (Hirano, Ishida, Ishihara)
Lesson 15: Analysis of the digests by agarose gel electrophoresis
           (Hirano, Ishida, Ishihara)

Text/Reference
Books,etc. Handouts, which include references, will be distributed in class.

PC or AV used in
Class,etc. 　

(More Details) PC (PowerPoint presentation)

Learning techniques to be incorporated 　

Suggestions on
Preparation and
Review Students are required to read the provided prints very carefully, and write down the experimental protocol for each experiment in their notebooks.

Requirements

Grading Method To receive credit, students must attend all lessons.
Students will be graded as follows:
i. Report and experimental notebook 70%
ii. Attitude toward the class 30%

Practical Experience

Summary of Practical Experience and Class Contents based on it

Message

Other

Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching.

Academic Year	2024Year	School/Graduate School	School of Integrated Arts and Sciences Department of Integrated Arts and Sciences
Lecture Code	ANL19001	Subject Classification	Specialized Education
Subject Name	生命科学実験法Ｃ
Subject Name （Katakana）	セイメイカガクジッケンホウC
Subject Name in English	Practical Methods in Life Sciences C
Instructor	ISHIDA ATSUHIKO,ISHIHARA YASUHIRO,HIRANO TETSUO
Instructor (Katakana)	イシダ　アツヒコ,イシハラ　ヤスヒロ,ヒラノ　テツオ
Campus	Higashi-Hiroshima	Semester/Term	3rd-Year, First Semester, 1Term
Days, Periods, and Classrooms	(1T) Thur5,Fri5：IAS B414
Lesson Style	Lecture	Lesson Style (More Details)
In this class students will develop their skills in biochemical and molecular biological experiments.
Credits	1.0	Class Hours/Week		Language of Instruction	J : Japanese
Course Level	3 : Undergraduate High-Intermediate
Course Area（Area）	26 : Biological and Life Sciences
Course Area（Discipline）	04 : Life Sciences
Eligible Students
Keywords	Protein, purification, enzyme activity, nucleic acid, plasmid DNA, transformation, restriction enzyme
Special Subject for Teacher Education		Special Subject
Class Status within Educational Program (Applicable only to targeted subjects for undergraduate students)
Criterion referenced Evaluation (Applicable only to targeted subjects for undergraduate students)	Integrated Arts and Sciences （Abilities and Skills）・The abilities and skills to summarize one's own research in reports or academic papers, and to deliver presentations at a seminar or research meetings, and to answer questions.
Class Objectives /Class Outline	This course enables students to master their skills in biochemical experiments through experiments in protein purification, fractionation of organelles, and enzymatic analysis. Basic experiments in molecular biology, such as transformation and PCR, are also carried out. Students will develop their abilities to handle various biochemical samples, such as proteins and nucleic acids.
Class Schedule	Lesson 1: Introduction to biochemical experiments (Ishida, Ishihara) Lesson 2: Protein purification by ion-exchange chromatography (Ishida, Ishihara) Lesson 3: Electrophoresis of proteins (Ishida, Ishihara) Lesson 4: Protein assay (Ishida, Ishihara) Lesson 5: Fractionation of various organelles from rat liver (Ishida, Ishihara) Lesson 6: Protein assay of the fractionated organelles (Ishida, Ishihara) Lesson 7: Enzyme activity assay (1) (Ishida, Ishihara) Lesson 8: Enzyme activity assay (2) (Ishida, Ishihara) Lesson 9: Introduction to molecular biology and preparation of reagents and buffers (Hirano, Ishida, Ishihara) Lesson 10: Transformation of E. coli (Hirano, Ishida, Ishihara) Lesson 11: Estimation of transformation efficiency, PCR, preparation of agarose gels (Hirano, Ishida, Ishihara) Lesson 12: Agarose gel electrophoresis of PCR products (Hirano, Ishida, Ishihara) Lesson 13: Preparation of plasmid DNAs (Hirano, Ishida, Ishihara) Lesson 14:Digestion of the plasmids by restriction enzymes (Hirano, Ishida, Ishihara) Lesson 15: Analysis of the digests by agarose gel electrophoresis (Hirano, Ishida, Ishihara)
Text/Reference Books,etc.	Handouts, which include references, will be distributed in class.
PC or AV used in Class,etc.
(More Details)	PC (PowerPoint presentation)
Learning techniques to be incorporated
Suggestions on Preparation and Review	Students are required to read the provided prints very carefully, and write down the experimental protocol for each experiment in their notebooks.
Requirements
Grading Method	To receive credit, students must attend all lessons. Students will be graded as follows: i. Report and experimental notebook 70% ii. Attitude toward the class 30%
Practical Experience
Summary of Practical Experience and Class Contents based on it
Message
Other
Please fill in the class improvement questionnaire which is carried out on all classes. Instructors will reflect on your feedback and utilize the information for improving their teaching.

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