Hiroshima University Syllabus

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Academic Year 2024Year School/Graduate School School of Integrated Arts and Sciences Department of Integrated Arts and Sciences
Lecture Code ANL19001 Subject Classification Specialized Education
Subject Name 生命科学実験法C
Subject Name
Subject Name in
Practical Methods in Life Sciences C
イシダ アツヒコ,イシハラ ヤスヒロ,ヒラノ テツオ
Campus Higashi-Hiroshima Semester/Term 3rd-Year,  First Semester,  1Term
Days, Periods, and Classrooms (1T) Thur5,Fri5:IAS B414
Lesson Style Lecture Lesson Style
(More Details)
In this class students will develop their skills in biochemical and molecular biological experiments. 
Credits 1.0 Class Hours/Week   Language of Instruction J : Japanese
Course Level 3 : Undergraduate High-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 04 : Life Sciences
Eligible Students
Keywords Protein, purification, enzyme activity, nucleic acid, plasmid DNA, transformation, restriction enzyme 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
(Applicable only to targeted subjects for undergraduate students)
Criterion referenced
(Applicable only to targeted subjects for undergraduate students)
Integrated Arts and Sciences
(Abilities and Skills)
・The abilities and skills to summarize one's own research in reports or academic papers, and to deliver presentations at a seminar or research meetings, and to answer questions. 
Class Objectives
/Class Outline
This course enables students to master their skills in biochemical experiments through experiments in protein purification, fractionation of organelles, and enzymatic analysis. Basic experiments in molecular biology, such as transformation and PCR, are also carried out.
Students will develop their abilities to handle various biochemical samples, such as proteins and nucleic acids. 
Class Schedule Lesson 1: Introduction to biochemical experiments
           (Ishida, Ishihara)
Lesson 2: Protein purification by ion-exchange chromatography
           (Ishida, Ishihara)
Lesson 3: Electrophoresis of proteins  (Ishida, Ishihara)
Lesson 4: Protein assay (Ishida, Ishihara)
Lesson 5: Fractionation of various organelles from rat liver
           (Ishida, Ishihara)
Lesson 6: Protein assay of the fractionated organelles
           (Ishida, Ishihara)
Lesson 7: Enzyme activity assay (1) (Ishida, Ishihara)
Lesson 8: Enzyme activity assay (2) (Ishida, Ishihara)
Lesson 9: Introduction to molecular biology and preparation of reagents and buffers  (Hirano, Ishida, Ishihara)
Lesson 10: Transformation of E. coli (Hirano, Ishida, Ishihara)
Lesson 11: Estimation of transformation efficiency, PCR, preparation of agarose gels  (Hirano, Ishida, Ishihara)
Lesson 12: Agarose gel electrophoresis of PCR products
           (Hirano, Ishida, Ishihara)
Lesson 13: Preparation of plasmid DNAs
           (Hirano, Ishida, Ishihara)
Lesson 14:Digestion of the plasmids by restriction enzymes
           (Hirano, Ishida, Ishihara)
Lesson 15: Analysis of the digests by agarose gel electrophoresis
           (Hirano, Ishida, Ishihara) 
Handouts, which include references, will be distributed in class. 
PC or AV used in
(More Details) PC (PowerPoint presentation) 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Students are required to read the provided prints very carefully, and write down the experimental protocol for each experiment in their notebooks. 
Grading Method To receive credit, students must attend all lessons.
Students will be graded as follows:
i. Report and experimental notebook 70%
ii. Attitude toward the class 30% 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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