Hiroshima University Syllabus

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Japanese
Academic Year 2022Year School/Graduate School Graduate School of Integrated Sciences for Life (Master's Course)
Lecture Code WI101600 Subject Classification Specialized Education
Subject Name ゲノム編集基礎演習
Subject Name
(Katakana)
ゲノムヘンシュウキソエンシュウ
Subject Name in
English
Basic Training of Genome Editing
Instructor SAKUMA TETSUSHI,SUGI TAKUMA,MIURA KENTO,HOSOBA KOSUKE,KUME KODAI,YAMAMOTO TAKASHI
Instructor
(Katakana)
サクマ テツシ,スギ タクマ,ミウラ ケント,ホソバ コウスケ,クメ コウダイ,ヤマモト タカシ
Campus Higashi-Hiroshima Semester/Term 1st-Year,  First Semester,  OutOfTerm(1st)
Days, Periods, and Classrooms (O1) Inte:See the timetable for detail.
Lesson Style Seminar Lesson Style
(More Details)
 
Experiment 
Credits 2.0 Class Hours/Week   Language of Instruction B : Japanese/English
Course Level 5 : Graduate Basic
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 04 : Life Sciences
Eligible Students Students in the Frontier Development Program for Genome Editing
Keywords  
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Program
 
Criterion referenced
Evaluation
 
Class Objectives
/Class Outline
Learn the basic technology of genome editing using cultured cells (how to treat human cultured cells, targeted mutagenesis using CRISPR-Cas9, and analytical methods of introduced mutations) 
Class Schedule Human cell culture
Introduction of CRISPR-Cas9 into human cells
Activity comparison between conventional and improved CRISPR-Cas9
Hands-on training of designing guide RNA and searching potential off-target sites
Hands-on training of designing gene knock-in and genotyping primers
Mutation analysis using heteroduplex mobility assay and Cel-I assay (on- and off-target analyses)

Report 
Text/Reference
Books,etc.
The course text will be distributed. 
PC or AV used in
Class,etc.
 
(More Details) PowerPoint and the like 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Review
Preparation and review are recommended according to the course text. 
Requirements Laptop is required for the course. 
Grading Method A grading will be given by evaluating the attitude and report. 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message This course will be conducted in Innovation Plaza of Hiroshima University. 
Other   
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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