Hiroshima University Syllabus

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Academic Year 2022Year School/Graduate School School of Integrated Arts and Sciences Department of Integrated Arts and Sciences
Lecture Code ANL13001 Subject Classification Specialized Education
Subject Name 生命科学実験法B
Subject Name
Subject Name in
Practical Methods in Life Sciences B
ヒコサカ アキラ,ワサキ ジュン,クガ ユカリ,ナカガワ ナオキ,カツヤマ チエ
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  4Term
Days, Periods, and Classrooms (4T) Thur5,Fri5:IAS B414
Lesson Style Lecture Lesson Style
(More Details)
Lecture is given using slides. 
Credits 1.0 Class Hours/Week   Language of Instruction J : Japanese
Course Level 3 : Undergraduate High-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 04 : Life Sciences
Eligible Students
Keywords Mycorrhiza, Symbiosis, Microbial culturing (bacteria & fungi), Sterilization Techniques, Staining, Microscopy, Multiple comparison analysis, Plant, Environmental Response, Nutrition, Rhizosphere, Enzyme, Bioinformatics, Programming, Genome, Molecular Evolution, Database, SDG_03, SDG_15 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Criterion referenced
Integrated Arts and Sciences
(Knowledge and Understanding)
・Knowledge and understanding of the importance and characteristics of each discipline and basic theoretical framework.
(Abilities and Skills)
・The ability and skills to specify necessary theories and methods for consideration of issues.
(Comprehensive Abilities)
・The ability to conduct research proactively by combining knowledge,  understanding, and skills for the tasks, based on flexible creativity and imagination. 
Class Objectives
/Class Outline
Several methodologies for environmental microbiology, plant physiology and bioinfomatic analysis which are required for "Laboratory Work in Life Sciences B" are explained. 
Class Schedule lesson1 Environmental microbiology lab using mycorrhiza 1: Sterilization techniques, agar culture media formation (YK)
lesson2 Environmental microbiology lab using mycorrhiza 2: Mycorrhizal root sampling and fixation, Isolation of bacteria and fungi from the roots (YK)
lesson3 Environmental microbiology lab using mycorrhiza 3: Staining roots (YK)
lesson4 Environmental microbiology lab using mycorrhiza 4: Observation of mycorrhiza, colonization rate measurements(YK)
lesson5 Environmental microbiology lab using mycorrhiza 5: Observation of isolated microbes, Multiple comparison analysis using R(YK)
lesson6 Laboratory work in plant physiology 1: Observation of plants grown under poor nutrient stresses (JW, NN)
lesson7 Laboratory work in plant physiology 2: Growth analysis, digestion, and enzyme activity (JW, NN)
lesson8 Laboratory work in plant physiology 3: Measurements of mineral content of plants
(JW, NN)
lesson9 Laboratory work in plant physiology 4: Quantification of mRNA for stress responsible genes (JW, NN)
lesson10 Laboratory work in plant physiology 5: Analysis of results (JW, NN)
lesson11 Bioinfomatic analysis 1 (AH)
lesson12 Bioinfomatic analysis 2 (AH)
lesson13 Bioinfomatic analysis 3 (AH)
lesson14 Bioinfomatic analysis 4 (AH)
lesson15 Bioinfomatic analysis 5 (AH)
Lecture notes describing the methodologies of experiments are provided. 
PC or AV used in
(More Details) Slides 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Read the lecture notes carefully. 
Grading Method Grade is determined based on the results of "Laboratory Work in Life Sciences B". 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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