Hiroshima University Syllabus

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Japanese
Academic Year 2021Year School/Graduate School School of Applied Biological Science
Lecture Code L5004004 Subject Classification Specialized Education
Subject Name 分子農学生命科学実験I
Subject Name
(Katakana)
ブンシノウガクセイメイカガクジッケンI
Subject Name in
English
Laboratory Work in Molecular Agro-life Science I
Instructor OHTA SHINJI,SAMBONGI YOSHIHIRO,FUJII SOTARO,OOMURA HISASHI
Instructor
(Katakana)
オオタ シンジ,サンボンギ ヨシヒロ,フジイ ソウタロウ,オオムラ ヒサシ
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  Intensive
Days, Periods, and Classrooms (Int) Inte:ABS A701 (Student Laboratory)
Lesson Style Experiment Lesson Style
(More Details)
 
Experiment
 
Credits 1.0 Class Hours/Week   Language on Instruction J : Japanese
Course Level 3 : Undergraduate High-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 01 : Agricultural Sciences
Eligible Students 2nd year students of the Faculty of Applied Biological Science
Keywords Solvent partition, organic synthesis, instrumental analysis, protein engineering 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Program
 
Criterion referenced
Evaluation
Molecular Agro-Life Science Program
(Abilities and Skills)
・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population
 
Class Objectives
/Class Outline
To learn basic technologies on the biological chemistry
To learn basic instrumental analyses
To learn basis of protein engineering 
Class Schedule 1) Guidance, Synthesis of bioactive compound
2) Purification of bioactive compound
3) Determination of purity
4) Solvent partition of organic compounds
5) Analyses of organic compounds by IR and TLC
6) Analyses of organic compounds by GC and HPLC
7) Collection of recombinant cytochrome c
8) Quantification of cytochrome c by absorption spectrum measurement and Bradford method
9) Check of cytochrome c expression by SDS-PAGE 
Text/Reference
Books,etc.
A textbook will be distributed through Bb9 system, etc. 
PC or AV used in
Class,etc.
 
(More Details) Textbook, writing on the blackboard, etc
 
Learning techniques to be incorporated  
Suggestions on
Preparation and
Review
Read the textbook.
 
Requirements  
Grading Method Experiments (~30%) and reports (~70%) 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message  
Other   
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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