Hiroshima University Syllabus

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Japanese
Academic Year 2020Year School/Graduate School Graduate School of Biomedical & Health Sciences (Doctor's course) Medicinal Sciences Major
Lecture Code TA010320 Subject Classification Specialized Education
Subject Name スタートアップ生命科学コースワーク
Subject Name
(Katakana)
スタートアップセイメイカガクコースワーク
Subject Name in
English
Start-Up Coursework of Biomedical Sciences
Instructor CHAYAMA HIROMI
Instructor
(Katakana)
チャヤマ ヒロミ
Campus Kasumi Semester/Term 2nd-Year,  First Semester,  First Semester
Days, Periods, and Classrooms (1st) Inte
Lesson Style Seminar Lesson Style
【More Details】
lecture style etc... 
Credits 2 Class Hours/Week   Language on Instruction B : Japanese/English
Course Level 7 : Graduate Special Studies
Course Area(Area) 27 : Health Sciences
Course Area(Discipline) 01 : Medical Sciences
Eligible Students  
Keywords  
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Program
 
Criterion referenced
Evaluation
 
Class Objectives
/Class Outline
Study the basic technique and knowledge on the biomedical and molecular biological research as a coursework 
Class Schedule Omnibus.
8  lecture.
1 "[Group A: Wed, May 13th 13:00-16:00], [Group B: Fri, May 8th 9:00-12:00]  
" Review of molecular biology, Practice of cell culturing. "Students will understand DNA,RNA and protein structures, molecular genetics and the
biochemistry of transcription and protein synthesis. In addition, they will do cell culturing." Building A, 2nd floor A201 You are required to provide your lab coat and writing implements.
2 "[Group A: Wed, May 20th 13:00-16:00], [Group B: Fri, May 15th 9:00-12:00]
" Transfection DNA Plasmid into Mammalian Cells Students can carry out basic cell-culture and transfection techniques properly and safely. Building A, 2nd floor A221 You are required to provide your lab coat and writing implements.
3 "[Group A: Wed, May 27th 13:00-16:00], [Group B: Fri, May 22nd 9:00-12:00]
" Purification of nucleic acid Students will learn about principle and technique of nucleic acid purification, reverse transcription and cDNA synthesis. Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.
4 "[Group A: Wed, June 3rd 13:00-16:00], [Group B: Fri, May 29th 9:00-12:00]
" PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 1 Students will search for primers for targeted genes and learn about principle and technique of PCR, agarose gel electrophoresis. In addition, Students will learn about principle and technique of mRNA expression level quantification by real time PCR. Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.
5 "[Group A: Wed, June 10th 13:00-16:00], [Group B: Fri, June 5th 9:00-12:00]
" PCR, agarose gel electrophoresis and quantification mRNA expression by real time PCR part 2 Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.
6 "[Group A: Tue, June 16th 9:00-17:00], [Group B: Thu, June 11th 9:00-17:00]
" Protein gel electrophoresis part1 Students will learn about principle and technique of protein gel electrophoresis using SDS-PAGE. In addition, they will set up gels and perform western blots. Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.
7 "[Group A: Wed, June 17th 13:00-16:00], [Group B: Fri, June 12th 9:00-12:00]
" Protein gel electrophoresis part2 Students will detect targeted protein expression using ECL reagents with the ChemiDoc imaging system. Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.
8 "[Group A: Wed, June 24th 13:00-16:00], [Group B: Fri, June 19th 9:00-12:00]
" Data analysis and review of the class Students will analyze data by T-TEST and report their image data by western blots. Building A, 2nd floor A201 You are required to provide your laptop and writing implements.
 
Text/Reference
Books,etc.
handout 
PC or AV used in
Class,etc.
laptop 
Suggestions on
Preparation and
Review
handout 
Requirements *You must choose either Group A or Group B. Please make sure the class date, time, venue and what you need for the class.
If you have further questions, please contact Professor Hiromi Chayama: habe@hiroshima-u.ac.jp. 
Grading Method ・attendance
 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message  
Other #1 and #8 classes will move to online format. I will give you an assignment in #2-#7 classes via email. 
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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