Hiroshima University Syllabus

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Japanese
Academic Year 2020Year School/Graduate School Graduate School of Biomedical & Health Sciences (Doctor's course) Oral Health Sciences Major
Lecture Code TA010410 Subject Classification Specialized Education
Subject Name アドバンスド生命科学コースワーク
Subject Name
(Katakana)
アドバンスドセイメイカガクコースワーク
Subject Name in
English
Advanced Coursework of Biomedical Sciences
Instructor CHAYAMA HIROMI
Instructor
(Katakana)
チャヤマ ヒロミ
Campus Kasumi Semester/Term 2nd-Year,  Second Semester,  Second Semester
Days, Periods, and Classrooms (2nd) Inte
Lesson Style Seminar Lesson Style
【More Details】
Lecture and discussion 
Credits 2.0 Class Hours/Week   Language on Instruction B : Japanese/English
Course Level 7 : Graduate Special Studies
Course Area(Area) 27 : Health Sciences
Course Area(Discipline) 01 : Medical Sciences
Eligible Students  
Keywords pharmacotherapy, pathophysiology, pathogenic mechanism, signal transduction 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Program
 
Criterion referenced
Evaluation
 
Class Objectives
/Class Outline
To develop students' understanding of how to conduct experimental research in the medical and biological fields, they will work on a mini-project in small groups. 
Class Schedule 1 Friday, October 9th 9:00-12:00 Cell Culturing and Drug Treatment Students can carry out basic cell-culture and drug treatment. Building A, 2nd floor A201 You are required to provide your lab coat and writing implements.


2 Friday, October 16th 9:00-12:00 Transfection, Cell Harvest and Bradford Assay Students will perform transfection techniques, harvest cells and perform protein quantification by Bradford method. Building A, 2nd floor A221 You are required to provide your lab coat and writing implements.


3 Friday, October 23rd 9:00-12:00 Luciferase assay Students will determine the transcriptional activity by the Luciferase Assay method. Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.


4 Friday, October 30th 9:00-12:00 Purification of Plasmid DNA Students willpPurify plasmid DNA by alkali-SDS method. Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.


5 Friday, November 6th 9:00-12:00 Primer design and real time PCR Students will design primers and quantify the expression level of the target gene (one of the genes induced by the drug treatment) by real time PCR. Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.


6 Thursday, November 12th 9:00-12:00 Western Blotting, Part 1 Students will detect the expression of target proteins (proteins phosphorylated by the drug treatment) by SDS-PAGE and western blotting. Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.


7 Friday, November 13th 9:00-12:00 Western Blotting, Part 2 Students will detect the expression of target proteins (proteins phosphorylated by the drug treatment) by SDS-PAGE and western blotting. Building A, 2nd floor A223 You are required to provide your lab coat and writing implements.


8 Friday, November 20th 9:00-12:00 Data analysis Students will visualize the results and evaluate the experimental results by statistical analysis of the changes in the expression of specific genes caused by the drug treatment. Students will discuss what happened in the cells by the drug treatment. Building A, 2nd floor A201 You are required to provide your laptop and writing implements.

 
Text/Reference
Books,etc.
Handouts 
PC or AV used in
Class,etc.
Power Point, Laptop 
Suggestions on
Preparation and
Review
Please read handouts. 
Requirements *Check the available dates.
*If you have any questions about these seminars, please contact with Hiromi Chayama-sensei.
e:mail:habe@hiroshima-u.ac.jp
 
Grading Method Attendance Rate, Reports 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message  
Other   
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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