Hiroshima University Syllabus

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Japanese
Academic Year 2020Year School/Graduate School School of Applied Biological Science
Lecture Code L5008004 Subject Classification Specialized Education
Subject Name 分子農学生命科学実験III
Subject Name
(Katakana)
ブンシノウガクセイメイカガクジッケンIII
Subject Name in
English
Laboratory Work in Molecular Agro-life ScienceIII
Instructor FUNATO KOUICHI,MATSUZAKI MEI,HORIUCHI HIROYUKI
Instructor
(Katakana)
フナト コウイチ,マツザキ メイ,ホリウチ ヒロユキ
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  Intensive
Days, Periods, and Classrooms (Int) Inte
Lesson Style Experiment Lesson Style
【More Details】
Experiment 
Credits 1 Class Hours/Week   Language on Instruction J : Japanese
Course Level 2 : Undergraduate Low-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 01 : Agricultural Sciences
Eligible Students  
Keywords cell engineering, cell culture, transfection, centrifugation, cell fractionation, microscopic observation, cell/organelle staining 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Program
 
Criterion referenced
Evaluation
Molecular Agro-Life Science Program
(Abilities and Skills)
・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population
 
Class Objectives
/Class Outline
The experimental basis to analyze cells is learned. 
Class Schedule lesson1
Guidance and cell culture
lesson2
Introduction of DNA into cells (yeast)
lesson3
Colony PCR for confirmation of gene knockout
lesson4
Isolation of knockout cells and observation of fluorescent proteins
lesson5
Understanding of reagents and laboratory equipment for animal cells
lesson6
Separation of several cells in blood
lesson7
Immunostaining of cultured cells
lesson8
Observation of animal tissues
lesson9
lesson10
lesson11
lesson12
lesson13
lesson14
lesson15 
Text/Reference
Books,etc.
A textbook will be distributed. 
PC or AV used in
Class,etc.
centrifuge, autoclave, fluorescence microscope, invert microscope, etc 
Suggestions on
Preparation and
Review
Read the textbook, which will be distributed on the first day, and write and submit a report. 
Requirements  
Grading Method Experiments and reports 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message  
Other   
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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