Hiroshima University Syllabus

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Japanese
Academic Year 2020Year School/Graduate School School of Applied Biological Science
Lecture Code L5006004 Subject Classification Specialized Education
Subject Name 分子農学生命科学実験II
Subject Name
(Katakana)
ブンシノウガクセイメイカガクジッケンII
Subject Name in
English
Laboratory Work in Molecular Agro-life ScienceII
Instructor KUNIYOSHI HISATO,TANAKA WAKANA,FUJIKAWA YUKICHI
Instructor
(Katakana)
クニヨシ ヒサト,タナカ ワカナ,フジカワ ユキチ
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  Intensive
Days, Periods, and Classrooms (Int) Inte
Lesson Style Experiment Lesson Style
【More Details】
Molecular Biological experiments. 
Credits 1.0 Class Hours/Week   Language on Instruction J : Japanese
Course Level 2 : Undergraduate Low-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 01 : Agricultural Sciences
Eligible Students  
Keywords RNA extraction, Reverse transcription, PCR, Subcloning, Sequence 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Program
 
Criterion referenced
Evaluation
Molecular Agro-Life Science Program
(Abilities and Skills)
・Intellectual ability and techniques in study fields regarding organic molecule, cell, individual organism, and population
 
Class Objectives
/Class Outline
The main aim of this class is to learn experimental techniques in molecular biology. 
Class Schedule lesson1: Introductioin.
lesson2: Preparation of reagents.
lesson3: RNA extraction.
lesson4: Reverse transcription, PCR.
lesson5: Electrophoresis.
lesson6: Purification of PCR products.
lesson7: Transformation.
lesson8: Preparation of plasmid DNA.
lesson9: Restriction enzyme digestion.
lesson10: Sequencing.
lesson11: Data analyses.
lesson12: Summary.

Report. 
Text/Reference
Books,etc.
A textbook for experiment will be given. 
PC or AV used in
Class,etc.
Experimental equipments. PC. 
Suggestions on
Preparation and
Review
Read the textbook for experiment. 
Requirements  
Grading Method Report(60%) &  attitude during experiment(40%). 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message  
Other   
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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