Hiroshima University Syllabus

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Japanese
Academic Year 2020Year School/Graduate School School of Science
Lecture Code HL030000 Subject Classification Specialized Education
Subject Name 生物科学基礎実験III
Subject Name
(Katakana)
セイブツカガクキソジッケン3
Subject Name in
English
Practice for Fundamental Biology III
Instructor SHIMAMURA MASAKI,TAKAHASHI HARUKO,SHIMADA HIROSHI,TAKAHASHI YOUSUKE,KIKUCHI YUTAKA,TSUDA MASATAKA,IDE HIROSHI,INOUE YUYA,SHIMODE SAYUMI,HOSOBA KOSUKE,MIURA IKUO,HANADA HIDEKI,NAKATSUBO KEIKO,MORIGUCHI KAZUKI,TAZAWA ICHIRO,SUZUKI ATSUSHI,NAKAJIMA KEISUKE,FUKAZAWA JUTAROU,FURUNO NOBUAKI,YAMAGUCHI TOMIO
Instructor
(Katakana)
シマムラ マサキ,タカハシ ハルコ,シマダ ヒロシ,タカハシ ヨウスケ,キクチ ユタカ,ツダ マサタカ,イデ ヒロシ,イノウエ ユウヤ,シモデ サユミ,ホソバ コウスケ,ミウラ イクオ,ハナダ ヒデキ,ナカツボ ケイコ,モリグチ カズキ,タザワ イチロウ,スズキ アツシ,ナカジマ ケイスケ,フカザワ ジュタロウ,フルノ ノブアキ,ヤマグチ トミオ
Campus Higashi-Hiroshima Semester/Term 3rd-Year,  First Semester,  First Semester
Days, Periods, and Classrooms (1st) Weds5-10,Fri5-10
Lesson Style Experiment Lesson Style
【More Details】
Experiments, Fieldworks, Use of chemicals 
Credits 6 Class Hours/Week   Language on Instruction J : Japanese
Course Level 3 : Undergraduate High-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 03 : Basic Biology
Eligible Students 3rd-year students of the Department of Biological Science
Keywords Developmental biology, Plant physiology, Molecular biology, Plant systematics, Animal Phylogenetics, Enzyme biochemistry 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Program
Students get the ability to conduct the experiments in Developmental biology, Plant physiology, Molecular biology, Plant systematics, Animal Phylogenetics, Enzyme biochemistry based on the basic knowledge. 
Criterion referenced
Evaluation
Biology
(Abilities and Skills)
・Based on basic knowledge which is already acquired, to obtain the following experimental skills in order to practice research: 1) Basic observation skills and skills to manage experiments. 2) Ability to record observed natural phenomena. 3) Ability to collect and assess data. 
Class Objectives
/Class Outline
Students will learn experimental procedures for developmental biology, plant physiology, animal physiology, plant systematics and cell biology as the basis of the study of biological sciences. 
Class Schedule 1. General guidance

2-6. Developmental biology
(1) Observation of early development of echinoderm
(2) Observation of early development of amphibian
(3) Analysis of expression patterns of morphogenetic genes during development

7-11. Plant physiology
(1) Organ regeneration of tobacco leaf disk by plant hormones
(2) Transient assay using protoplast of Arabidopsis cultured cell
(3) The effect of plant hormone to plant growth and development
(4) Feedback regulation of plant hormone

12-16. Molecular biology
(1) Guidance for the experimental schedule, preparation of media and reagents, and construction of a recombinant plasmid
(2) Transformation of E. coli cells by introduction of the recombinant plasmid
(3) Analysis of the recombinant plasmid (1)
(4) Analysis of the recombinant plasmid (2)
(5) Expression analysis of a gene in the recombinant plasmid, Discussion

22-26. Animal Phylogenetics
(1) DNA extraction from animal tissue and PCR
(2) Purification of PCR product
(3) DNA sequencing
(4) Phylogenetic tree reconstruction based on molecular data -1
(5) Phylogenetic tree reconstruction based on molecular data -2


27-30.Enzyme biochemistry
(1) Guidance, Kinetic analysis of alkaline phosphatase.1
(2) Kinetic analysis of alkaline phosphatase.2
(3) Educational training of RI
(4) RI-labeling of DNA terminus by polynucleotide kinase, Discussion
 
Text/Reference
Books,etc.
Textbook for "Practice for Fundamental Biology"
Some reference materials may be distributed for each experiment.
 
PC or AV used in
Class,etc.
Textbook, PC, research equipments 
Suggestions on
Preparation and
Review
Textbook for "Practice for Fundamental Biology" should be read before and after each experiment.
Report of each experiment should be prepared immediately after experiment.
Each report should include (1) purpose, (2) experimental procedures, (3) results and (4) discussions.
 
Requirements Students have to attend all experiments and any truancy is not allowed. 
Grading Method The grade will be evaluated by reports (80%) and attitude (20%). 
Practical Experience  
Summary of Practical Experience and Class Contents based on it  
Message Because each experiment may involve the use of hazardous materials, students need to pay enough attention to the experimental procedures. 
Other   
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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