Hiroshima University Syllabus

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Japanese
Academic Year 2019Year School/Graduate School School of Science
Lecture Code HL020000 Subject Classification Specialized Education
Subject Name 生物科学基礎実験II
Subject Name
(Katakana)
セイブツカガクキソジッケン2
Subject Name in
English
Practice for Fundamental Biology II
Instructor SAKAMOTO NAOAKI,IGAWA TAKESHI,KUSABA MAKOTO,OKUMURA MISAKO,KOZUKA TOSHIAKI,MIURA IKUO,MORISHITA FUMIHIRO,TAKASE MINORU,HANADA HIDEKI,NAKATSUBO KEIKO,TSUBOTA HIROMI,HAMAO KOZUE,UEKI TATSUYA
Instructor
(Katakana)
サカモト ナオアキ,イガワ タケシ,クサバ マコト,オクムラ ミサコ,コヅカ トシアキ,ミウラ イクオ,モリシタ フミヒロ,タカセ ミノル,ハナダ ヒデキ,ナカツボ ケイコ,ツボタ ヒロミ,ハマオ コズエ,ウエキ タツヤ
Campus Higashi-Hiroshima Semester/Term 2nd-Year,  Second Semester,  Second Semester
Days, Periods, and Classrooms (2nd) Tues1-4,Weds1-4:IMC-Main Computer Rm
Lesson Style Experiment Lesson Style
【More Details】
Experiment, field work, use of chemicals 
Credits 4 Class Hours/Week   Language on Instruction J : Japanese
Course Level 3 : Undergraduate High-Intermediate
Course Area(Area) 26 : Biological and Life Sciences
Course Area(Discipline) 26 : Basic Biology
Eligible Students 2nd-year students of the Department of Biological Science
Keywords structure and function of cell, gene mapping and chromosome, animal response, bioinformatics, gene and transformation, sea urchin development,Practical work experience 
Special Subject for Teacher Education   Special Subject  
Class Status
within Educational
Program
Students learn skills for biological observation and experimental manipulation and abilities to evaluate experimental results and data with scientific view points. 
Criterion referenced
Evaluation
Biology
(Abilities and Skills)
・Based on basic knowledge which is already acquired, to obtain the following experimental skills in order to practice research: 1) Basic observation skills and skills to manage experiments. 2) Ability to record observed natural phenomena. 3) Ability to collect and assess data. 
Class Objectives
/Class Outline
Students will learn experimental procedures for basic molecular biology, basic developmental biology, cell biology, genetics, animal physiology and bioinformatics as the basis of the study of biological sciences. 
Class Schedule 1. General guidance (all instructors)
2. Cell biology (1) Design of experimental plan (HAMAO KOZUE, TAKASE MINORU, OKUMURA MISAKO)
3. Cell biology (2) Indirect immunofluorescent staining (HAMAO KOZUE, TAKASE MINORU, OKUMURA MISAKO)
4. Cell biology (3) Observation by fluorescent microscopy (HAMAO KOZUE, TAKASE MINORU, OKUMURA MISAKO)
5. Cell biology (4) Immunoprecipitation and electrophoresis (HAMAO KOZUE, TAKASE MINORU, OKUMURA MISAKO)
6. Cell biology (5) Immunoblotting (HAMAO KOZUE, TAKASE MINORU, OKUMURA MISAKO)
7. Cell biology (6) Discussion (HAMAO KOZUE, TAKASE MINORU, OKUMURA MISAKO)
8. Bioinformatics (1) Retrieval of references (MORISHITA FUMIHIRO, IGAWA TAKESHI)
9. Bioinformatics (2) Homology search on protein and nucleotide databases (MORISHITA FUMIHIRO, IGAWA TAKESHI)
10. Bioinformatics (3) Protein modeling (MORISHITA FUMIHIRO, IGAWA TAKESHI)
11. Bioinformatics (4) Basis of biological statistics (TSUBOTA HIROMI, MIURA IKUO)
12. Bioinformatics (5) Basis of R and basic statistics (TSUBOTA HIROMI, MIURA IKUO)
13. Bioinformatics (6) Multivariate analysis by R (TSUBOTA HIROMI, MIURA IKUO)
14. Animal physiology (1) Responses of the invertebrates (UEKI TATSUYA, MORISHITA FUMIHIRO, TAKASE MINORU)
15. Animal physiology (2) Hormonal regulation (UEKI TATSUYA, MORISHITA FUMIHIRO, TAKASE MINORU)
16. Animal physiology (3) Neural regulation (UEKI TATSUYA, MORISHITA FUMIHIRO, TAKASE MINORU)
17. Animal physiology (4) Discussion and presentation (UEKI TATSUYA, MORISHITA FUMIHIRO, TAKASE MINORU)
18. Genetics (1) Observation of animal chromosomes (I) (MIURA IKUO, HANADA HIDEKI, IGAWA TAKESHI)
19. Genetics (2) Observation of animal chromosomes (II) (MIURA IKUO, HANADA HIDEKI, IGAWA TAKESHI)
20. Genetics (3) Discussion (MIURA IKUO, HANADA HIDEKI, IGAWA TAKESHI)
21. Basic molecular biology (1) Preparation of reagents (SAKAMOTO NAOAKI, NAKATSUBO KEIKO, HIROSHI OCHIAI)
22. Basic molecular biology (2) Transformation (SAKAMOTO NAOAKI, NAKATSUBO KEIKO, HIROSHI OCHIAI)
23. Basic molecular biology (3) Restriction enzyme digestion and agarose gel electrophoresis (SAKAMOTO NAOAKI, NAKATSUBO KEIKO, HIROSHI OCHIAI)
24. Basic molecular biology (4) Extraction of plasmid DNA (SAKAMOTO NAOAKI, NAKATSUBO KEIKO, HIROSHI OCHIAI)
25. Genetics (4) Observation and selection of Arabidopsis mutants (KUSABA MAKOTO, KOZUKA TOSHIAKI)
26. Genetics (5) Gene mapping (1) (KUSABA MAKOTO, KOZUKA TOSHIAKI)
27. Genetics (6) Gene mapping (2) (KUSABA MAKOTO, KOZUKA TOSHIAKI)
28. Basic developmental biology (1) Observation of sea urchin development (I) (SAKAMOTO NAOAKI, NAKATSUBO KEIKO, HIROSHI OCHIAI)
29. Basic developmental biology (2) Observation of sea urchin development (II) (SAKAMOTO NAOAKI, NAKATSUBO KEIKO, HIROSHI OCHIAI)
30. Basic developmental biology (3) Extraction of RNA from sea urchin embryos (SAKAMOTO NAOAKI, NAKATSUBO KEIKO, HIROSHI OCHIAI) 
Text/Reference
Books,etc.
Textbook for "Practice for Fundamental Biology"
Some reference materials may be distributed for each experiment. 
PC or AV used in
Class,etc.
Textbook, PC, research equipments 
Suggestions on
Preparation and
Review
Textbook for "Practice for Fundamental Biology" should be read before and after each experiment.
Report of each experiment should be prepared immediately after experiment.
Each report should include (1) purpose, (2) experimental procedures, (3) results and (4) discussions. 
Requirements Students have to attend all experiments and any truancy is not allowed. 
Grading Method The grade will be evaluated by reports (80%) and attitude (20%). 
Message Because each experiment may involve the use of hazardous materials, students need to pay enough attention to the experimental procedures. 
Other   
Please fill in the class improvement questionnaire which is carried out on all classes.
Instructors will reflect on your feedback and utilize the information for improving their teaching. 
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